2009年江苏省徐州市副溶血性弧菌病原学特征的分析研究

目的 了解徐州市副溶血性弧菌(VP)分布及病原学特征。 方法 对样品进行常规检测,血清学分型,药敏试验; PCR技术检测VP的TDH、TRH毒力基因。 结果 徐州市水产品VP检出率为57.50%(23/40),其中2株trh基因(+); 23株VP中3株 (7.50%,3/40)未定血清型,其余20株分布在7个血清型,有O1、2、3、4、5、10、11型; 其中O1、O4、O5构成比为65.22%(15/23),是主要流行血清型。食源性腹泻患者粪便中VP检出率为8.00%(4/50),其中2株为O3:K6血清型且tdh基因(+)。水产品中VP的trh基因阳性率为8.70%(2/23),未检出tdh基因;食源性腹泻标本检出菌tdh基因阳性率为50.00%,trh基因未检出。水产品中检出的VP主要对替卡西林、阿莫西林、妥布霉素、头孢呋辛、头孢西丁耐药;腹泻患者粪便中检出的VP主要对阿莫西林和替卡西林耐药。 结论 徐州市水产品VP携带率高,食源性腹泻患者粪便中检出VP, 且携带毒力基因; VP耐药情况较严重;不同病原学特征的VP引起的患者临床症状无明显差别。VP的防治工作中应进一步加强监测其血清型、耐药性以及携带毒力基因的情况;对腹泻患者的临床症状和VP的病原学特征之间的关系需进一步研究。

Etiological analysis of Vibrio parahaemolyticus in Xuzhou city in Jiangsu province,2009

Objective To investigate the etiological characteristics of Vibrio parahaemolyticus(VP) in Xuzhou.Methods VP was detected in the samples collected according to GB/T 4789.7,the serotyping of isolated VP strains was conducted,drug susceptibility test was performed for these isolates and virulence genes of tdh and trh were detected by using PCR.Results Twenty three VP strains were detected in 40 aquatic products(57.50%),2 of them were trh gene positive.Of 23 VP strains,3 were not specified in serotype,the othe...