筋骨草水煎液对急性肺炎的保护作用研究

目的:研究筋骨草水煎液对小鼠急性肺炎的保护作用及机制。方法:用LPS吸入法建立急性肺炎模型,设空白对照组、模型组、筋骨草水煎液组和地塞米松组。观察小鼠肺组织病理,测定肺指数、肺湿/干比,采用酶联免疫吸附试验法检测肺泡灌洗液中肿瘤坏死因子-α、白细胞介素-1β的含量。结果:筋骨草组与模型组比较减轻了急性肺炎小鼠肺组织病变,数据由SPSS 13.0软件分析,小鼠肺指数、肺湿干比明显降低,差异显著(P<0.05),降低肺泡灌洗液中TNF-α、IL-1β的含量,差异显著(P<0.05)。结论:筋骨草水煎液有一定的抗炎作用,可以缓解小鼠急性肺炎症状,其机制可能与降低TNF-α、IL-1β的含量有关。

Study on protective effect of water decotion of Ajuga decumbens on acute lung inflammation in mice

Objective:To study the protective effects of aqueous extract of Ajuga decumbens on acute lung inflammation induced by lipopolysaccharide(LPS) and the molecular mechanism of protection in mice.Methods:Models of acute lung inflammation were established by inhalation of LPS,mice were randomly divided into control group,model group,Ajuga decumbens group and dexamethasone group.Pathological changes in lung tissues of mice were observed,lung index(LI) and lung dry weight and wet weight ratio(W/D) were detected,TNF-α、IL-1β in BALF were measured by ELISA method.Results:The different concentration of aqueous extract of Ajuga decumbens could decrease pathological changes of lung tissues in acute lung inflammation mice compared with the model group.The data was analysis by SPSS13.0 software,LI,W/D and the content of TNF-α,IL-1β in BALF were significantly decreased,there were obvious differences(P<0.05).Conclusion:The aqueous extract of Ajuga decumbens has definite anti-inflammatory effect,can alleviate the symptoms of acute pneumonia in mice,and its mechanism may be related to the reduction of TNF-α and IL-1β content.