| 姜黄素对HepG2细胞固醇调控元件结合蛋白-2表达的影响 |
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| 引用本文： | 窦晓兵,陈庆,王宇浩,豆敏华,范春雷.姜黄素对HepG2细胞固醇调控元件结合蛋白-2表达的影响[J].环球中医药,2010,3(3):183-186. |
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| 作者姓名： | 窦晓兵 陈庆 王宇浩 豆敏华 范春雷 |
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| 作者单位： | 浙江中医药大学生命科学学院,杭州市,310053 |
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| 基金项目： | 国家自然科学基金，浙江省新苗人才计划项目，浙江中医药大学资助项目 |
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| 摘 要： | 目的研究姜黄素对人肝癌细胞株(Human hepatocellular liver carcinoma cell line,HepG2)固醇调控元件结合蛋白-2(sterol regulatory element-binding protein-2,SREBP-2)基因表达的影响,从信号调控通路水平进一步探讨姜黄素的降胆固醇作用机理。方法分别利用蛋白免疫印迹和实时荧光定量聚合酶链反应(Polymerase Chain Reactionm,PCR)技术研究姜黄素对HepG2细胞SREBP-2核片段碱性螺旋-环-螺旋蛋白(basic helix-loop-helix protein,bHLH)表达及SREBP-2 mRNA的影响。结果20 mol/L姜黄素可以促进HepG2细胞SREBP-2核片段bHLH增多,而对SREBP-2mRNA表达无影响。结论姜黄素可以通过促进HepG2细胞SREBP-2核片段bHLH的增多,上调低密度脂蛋白受体(low density lipoprotein receptor,LDL-R)的表达,起到降低血清胆固醇的作用。
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| 关 键 词： | 姜黄素 人肝癌细胞株 固醇调控元件结合蛋白-2 碱性螺旋-环-螺旋蛋白 |
The effect of curcumin on the expression of SREBP-2 in HepG2 |
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| Institution: | DOU Xiao-bing , CHEN Qing , WANG Yu-hao, et al. (Life Science College, Zhejiang Chinese Medical University, Hangzhou 310053, China) |
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| Abstract: | Objective To investigate the effects of curcumin on SREBP-2 expression in the human hepatoma cell line HepG2 and find out the mechanism of curcumin in lower-cholestrol at the SREBPs regu- lation pathway level. Methods Western blotting and Real time PCR was used to identify the effect of curcumin on the expression of SREBP-2 in the HepG2. Results The nuclear segment of active SREBP-2 （bHLH） was elevated after 20 mol/L curcumin treatment in the HepG2 by Western blotting. Conclusion Curcumin up-regulates the expression of the SREBP-2 in the HepG2. |
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| Keywords: | Curcumin HepG2 SREBP-2 bHLH |
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