纳米氧化铜对小白鼠的急性毒性及蓄积毒性试验研究

目的:观察纳米氧化铜对小白鼠的急性毒性及蓄积毒性,为临床提供依据。方法:1.急性毒性试验:给试验小鼠灌服不同浓度的纳米氧化铜混悬液,观察14 d内小鼠的活动情况及死亡情况,测定小鼠灌胃的LD50,采用改良寇氏公式计算LD50及可信限;2.蓄积毒性试验:依据20 d蓄积毒性试验法设计,取20~22 g小白鼠,共设5组,每组10只,雌雄各半,第1组为空白对照组,给试验小鼠灌服不同浓度的纳米氧化铜混悬液分别为28.90(1/20LD50)、57.81(1/10LD50)、115.62(1/5LD50)和298.05(1/2LD50)mg/(kg.bw)剂量,每日灌胃1次,连续灌胃20 d,通过对小白鼠灌服纳米氧化铜进行了蓄积毒性试验。结果:1.急性毒性试验:纳米氧化铜对小白鼠的LD50为578.09 mg/(kg.bw),其95%可信限343.92~971.63 mg/(kg.bw)。2.蓄积毒性试验:试验结果表明0和1/20LD50剂量组小白鼠全部存活,1/2LD50剂量组10只小白鼠全部死亡;1/5LD50剂量组小白鼠死亡5只,存活5只;1/10LD50剂量组小白鼠死亡2只,存活8只。结论:高剂量纳米氧化铜可对小鼠的肾脏、小肠、肝脏等组织器官造成广泛性损伤,并引起严重的淤血、出血等病理变化。依据判定标准,证明纳米氧化铜对小白鼠有中等蓄积毒性作用。

Acute Toxicity and Accumulate Toxicity Test of Nano-CuO in Mice

Objecttive:to observe the acute and accumulate toxicity of nano-CuO to provide basis for clinic use.Methods:1.Acute toxicity test:activity condition and death condition were observed within fourteen days with different concentration of solutions of nano-CuO,and the method of krber was used to determine LD50 of nano-CuO and confidence level;2.Accumulate toxicity test:based on the accumulate toxicity of 20 days' design.50 of 20～22 g Kunming mice were divided into 5 groups randonmly.The first group was control...