猴肝组织和人类上皮细胞联合检测血清抗核抗体的临床应用和评价

目的:对猴肝组织和人类上皮细胞(细胞)检测血清抗核抗体(Hep-2ANA)进行临床应用和评价。方法:以猴肝组织和细胞为基质,采用间接免疫荧光法()检测例结缔组织病患者,例非结缔组织病患者和例健康人血Hep-2IFA1188050清。ANA结果:细胞检测阳性率高于猴肝组织检测阳性率,且两种基质单独检测阳性率差别具有显著性Hep-2ANAANAANA(P),两种基质联合检测结缔组织病组与非结缔组织病及健康人组血清阳性率差别均具有高度显著性(<0.05ANAP< ),非结缔组织病与健康人血清阳性率差别无显著性(0.001ANAP)。滴度的观察对疾病的诊断有一定的意义。>0.05ANA 结论:检测应对每一份血清进行猴肝组织和细胞的联合检测。ANAHep-2

Clinical evaluation by monkey liver tissue and epithelial cells for detection of antinuclear antibodies in serum

Objective: To evaluate monkey liver tissue and human epithelial cells (Hep-2) as substrates for detection of antinuclear antibodies(ANA) in clinical use. Methods: Monkey liver tissue and Hep-2 cells were compared as substrates for detection of ANA in the serum of 118 patients with different connective tissue disease (CTD), 80 patients with no CTD and 50 normal subjects, using indirect immunofluorescence assay. Results: ANA on Hep-2 cells produced more positive results than ANA on monkey liver tissue, and the difference between them was statistically significant(P<0.05). Compared with the groups of non-CTD and normal, the positive rate of ANA on monkey liver tissue and Hep-2 cell in the groups of CTD was significantly different (P<0.001), and there was no significant difference between the groups of non-CTD and normal (P>0.05). The observation of ANA titer is helpful for diagnosis of CTD. Conclusion: ANA should be detected by Monkey liver tissue and Hep-2 cells.