PDTC对高糖培养大鼠肾成纤维细胞中MCP-1表达的影响

目的:探讨核因子-κB(NF-κB)抑制剂吡咯烷二硫氨基甲酸(PDTC)对高糖培养大鼠肾成纤维细胞(NRK)中单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:将NRK分4组进行体外培养:(1)正常组:5.6mmol/L的葡萄糖;(2)高糖组:30mmol/L葡萄糖;(3)高糖+PDTC1组:30mmo/L葡萄糖+5μmol/LPDTC;(4)高糖+PDTC2组:30mmo/L葡萄糖+10μmol/LPDTC,分别于培养24h、48h取各组NRK采用RT-PCR检测其MCP-1mRNA表达水平,采用WesternBlot检测MCP-1蛋白表达水平。结果:与正常组相比,高糖组MCP-1mRNA和蛋白表达水平显著升高(P<0.05),不同浓度PDTC干预后,MCP-1mRNA和蛋白表达水平显著下降(P<0.05),且随PDTC浓度增大,下降更明显。结论:高糖可使NRK中MCP-1表达增高,PDTC能抑制NRK中MCP-1表达。

Effect of PDTC on MCP-1 Induced by High Glucose of Rat Kidney Fibroblasts

Objective:To explore the effect of PDTC on MCP-1 induced by high glucose of rat kidney fibroblasts.Method:NRK cells were divided into four groups,and then cultured in vitro:(1)normal groups:5.6mmol/L glucose;(2)high glucose:30mmol/L glucose;(3)high glucose+PDTC 1 group:30mmol/L glucose+5μmol/L PDTC;(4)high glucose+PDTC 2 group:30mmol/L glucose+10μmol/L PDTC,the expression of MCP-1 mRNA and protein were measured by RT-PCR and western blot after 24h,48h,respectively.Results:Compared with normal groups,the MCP-1 mRNA was remarkabley increased in a time-dose dependent manner exposed to high glucose(P<0.05);the MCP-1 mRNA and protein were remarkabley decreased exposed to different concentrations of PDTC(P<0.05).Conclusion:The inhibitor of NF-κB,PDTC is able to downregulate the expression of MCP-1.