| 全脊髓匀浆建立豚鼠实验性自身免疫性脑脊髓炎模型 |
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| 引用本文： | 荣本兵,李作孝.全脊髓匀浆建立豚鼠实验性自身免疫性脑脊髓炎模型[J].泸州医学院学报,2007,30(2):104-106. |
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| 作者姓名： | 荣本兵 李作孝 |
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| 作者单位： | 泸州医学院附属医院神经内科,四川泸州,646000 |
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| 摘 要： | 目的:用全脊髓匀浆(粗制髓鞘碱性蛋白)建立豚鼠实验性自身免疫性脑脊髓炎(EAE)模型.方法:Wistar大鼠全脊髓匀浆 完全福氏完全佐剂(CFA)免疫豚鼠,观察豚鼠的临床症状和中枢神经系统(CNS)的病理改变.结果:实验豚鼠均表现典型的EAE症状;光镜观察见CNS血管周围炎性细胞浸润和白质脱髓鞘改变.结论:全脊髓匀浆加完全福氏完全佐剂成功的诱导了豚鼠的EAE模型,具有模型稳定、发病率高、动物廉价易得的优点,是研究多发性硬化理想的动物模型.
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| 关 键 词： | 实验性自身免疫性脑脊髓炎 髓鞘碱性蛋白 自身免疫性疾病 豚鼠 |
| 文章编号： | 1000-2669(2007)2-0104-03 |
| 修稿时间： | 09 14 2006 12:00AM |
ESTABLISHMENT OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MODEL ON GUINEA PIG |
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| Rong Benbing, et al.ESTABLISHMENT OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS MODEL ON GUINEA PIG[J].Journal of Luzhou Medical College,2007,30(2):104-106. |
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| Authors: | Rong Benbing |
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| Institution: | Department of Neurology, The Affdiated Hospital of Luzhou Medical College |
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| Abstract: | Objective:To establish the experimental autoimmune encephalomyelitis(EAE) model on Guinea Pig with pan-spinal cord homogenate(coarse myelin basic protein,cMBP).Methods:The Guinea Pig was immunized with Wistar rat pan-spinal cord homogenate Freund's complete adjuvant(CFA).The clinical symptoms and histopathologic changes of central nervous system were observed.Results:The Guinea Pigs in experimental group all demonstrated the typical symptoms of EAE with an incidence of 100%.The inflammatory cuff around small blood vessels,perivascular inflammatory cell infiltration and demyelination in white matter were observed through optical microscope.Conclusion:The EAE model of Guinea Pig was induced successfully,the model was stable and with high incidence;besides the Guinea Pig was cheap and easy to get.Thus this model might be an ideal animal model to study multiple sclerosis. |
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| Keywords: | EAE MBP Autoimmune disease Guinea Pig |
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