罗格列酮对高糖大鼠肾小管上皮细胞表达转化生长因子β1和纤维连接蛋白的影响

目的:观察罗格列酮(rosiglitazone,RGZ)对高糖环境下大鼠肾小管上皮细胞(NRK52E)转化生长因子β1(TGF_β1)和纤维连接蛋白(FN)表达的影响,初步探讨罗格列酮对糖尿病肾病的保护作用。方法:体外培养大鼠肾小管上皮细胞,分成正常对照组NG(含1000mg/LD_葡萄糖)、高糖组HG(含4500mg/LD_葡萄糖)、HG+RGZ(5μmol/L)组、HG+RGZ(10μmol/L)组和HG+RGZ(15μmol/L)组,用蛋白印迹的方法(Westernblotting)检测TGF_β1和FN蛋白表达的改变。结果:HG组细胞TGF_β1和FN的蛋白水平较NG组显著升高,加入罗格列酮后,TGF_β1和FN的表达较HG组降低。结论:罗格列酮可以抑制高糖诱导的小管细胞TGF_β1和FN高表达,具有一定的肾保护作用。

Effects of rosiglitazone on the expression of transforming growth factor β1 and fibronectin in rat renal tubular epithelial cells cultured in high glucose

Objective: To investigate the protective effects of rosiglitazone on rat renal tubular epithelial cells(NRK52E) damaged by high glucose.Methods: NRK52E cells were cultured in vitro,and were divided into five groups: normal glucose group(NG,1000 mg/L D-glucose),high glucose group(HG,4500 mg/L D-glucose),HG+RGZ(5μmol/L),HG+RGZ(10 μmol/L) and HG+RGZ(15 μmol/L).The expression of TGF-β1 and FN was examined by Western blotting.Results: Compared with NG group,expression of TGF-β1 and FN increased significantly in HG group,rosiglitazone inhibited those changes.Conclusion: Rosiglitazone can reduce the overexpression of TGF-β1 and FN induced by high glouse,has protective effects on kidney.