| 三氧化二砷对GLC-82细胞的增殖抑制机制 |
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| 引用本文： | 刘加军,伍新尧,王江峰,潘祥林. 三氧化二砷对GLC-82细胞的增殖抑制机制[J]. 肿瘤学杂志, 2003, 9(6): 347-349 |
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| 作者姓名： | 刘加军 伍新尧 王江峰 潘祥林 |
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| 作者单位： | 1. 中山大学中山医学院法医物证学实验室 广东 广州 510080
2. 山东大学第二医院血液肿瘤中心,山东,济南,250033 |
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| 摘 要： | [目的] 探讨三氧化二砷对肺癌细胞株GLC-82细胞的增殖抑制作用及其作用机制。[方法] 以不同浓度的三氧化二砷作用于体外培养的GLC-82细胞，MTT法检测细胞生长抑制率，应用流式细胞仪及Hoechst 33258荧光染色法观察细胞凋亡，TRAP-PCR-ELISA法检测细胞凋亡前后的端粒酶活性。[结果] 三氧化二砷可显著降低GLC-82细胞的端粒酶活性，抑制细胞的生长，诱导细胞发生凋亡。并呈现出明显的量-效与时-效关系。[结论] 三氧化二砷对肺癌细胞株GLC-82具有明显的增殖抑制作用。降低细胞端粒酶活性，诱导细胞发生调亡，可能是其重要作用机制之一。
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| 关 键 词： | 三氧化二砷 肺肿瘤 端粒酶 细胞凋亡 |
| 文章编号： | 1671-170X(2003)06-0347-03 |
| 修稿时间： | 2003-10-28 |
The Mechanism of Proliferation Inhibit with Arsenic Trioxide on GLC-82 Cells |
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| LIU Jia-jun,WU Xin-yao,WANG Jiang-feng,et al.. The Mechanism of Proliferation Inhibit with Arsenic Trioxide on GLC-82 Cells[J]. Journal of Chinese Oncology, 2003, 9(6): 347-349 |
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| Authors: | LIU Jia-jun WU Xin-yao WANG Jiang-feng et al. |
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| Abstract: | To investigate the i nhibitory effect of Arsenic trioxide on GLC-82cells(lung cancer strain)and its m echanism.GLC-82cells in culture medium in vitro were given different c oncentrations of Arsenic trioxide.The inhibitory ratio of the cells were measure d by MTT assay,apoptosis was obsevered by flow cytometry(FCM)and hoechst33258flu orescence staining,and the activity of telomerase was detected using TRAP-PCR-ELISA before and after apoptosis occurred.Arsenic trioxide could decrea se the activity of telomerase,as well as cause apoptosis and inhibit the growth of GLC-82cells significantly.The suppression was both in time-and dose-depend ent manner.[Conclusion]Arsenic trioxide has anti-proliferation effect on lung c ancer GLC-82cells in vitro,inducing apoptosis and decreasing the activity of te lomerase.It may be one of the most important mechanisms. |
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| Keywords: | arsenic trioxi de lung neoplasms telomerase apoptosis |
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