三种方法联合检测避免梅毒诊断的假阳性结果

目的探讨用梅毒螺旋体抗体酶联免疫吸附试验（TP-ELISA）联合梅毒甲苯胺红不加热血清试验（TRUST）和梅毒螺旋体明胶凝集试验（TPPA）的方法避免TP-ELISA法筛查梅毒抗体的假阳性结果。方法用TP-ELISA法作为临床标本的梅毒筛查实验,对阳性标本进一步作TRUST和TPPA检测。结果 TP-ELlSA法检出114例梅毒阳性标本,TPPA确证阳性111例,阳性符合率为97.36%（111/114）;其中27例TRUST阳性,TPPA确证试验均为阳性,阳性符合率为100%;97例TRUST阴性,TPPA确证阳性94例,阴性3例,不符合率为2.64%。结论 TP-ELISA法筛查梅毒存在一定比例的假阳性结果,对TP-ELlSA法测出的阳性标本先做TRUST试验,再用TPPA确证,阳性结果报告梅毒抗体阳性,阴性结果报告梅毒抗体阴性,这样能有效避免单一TP-ELISA法的假阳性结果。

Combining three ways to avoid the false positive of screening Treponema pallidum

Objective To investigate a method for avoiding the false positive of screening syphilis by Treponema pallidum antibody enzyme linked immunosorbent assay(TP-ELISA) though combining TP-ELISA with toluidine red unheated serum test(TRUST) and Treponema pallidum panicle agglutination test(TPPA).Methods The clinical samples were screened syphilis by TP-ELISA first,then the positive samples were further tested by TRUST and TPPA.Results There were 114 positive samples tested by TP-ELISA.Among them the number of the same result tested by TPPA was 111.The positive coincidence rate was 97.36%.Among the 111 positive samples,27 positive samples tested by TRUST were also positive tested by TPPA.The positive coincidence rate was 100%.There were 94 positive samples tested by TPPA among the remaining 97 negative samples tested by TRUST.The number of negative samples was 3.The mismatch rate was 2.64%.Conclusions The false positive result tested syphilis by TP-ELISA exists objectively.The positive samples from TP-ELISA were tested by TRUST and then were ascertained by TPPA.The positive result from TPPA can be diagnosed that syphilis antibody was positive.The negative result from TPPA can be diagnosed that syphilis antibody was negative.