短发夹RNA沉默S100A4基因对乳腺癌MCF-7细胞体外增殖和迁移力的抑制

目的观察靶向S100A4的shRNA对MCF-7细胞增殖和迁移力的影响。方法构建S100A4基因特异性shRNA表达载体,使用QRT-PCR和Western blot检测转染后MCF-7细胞中S100A4的表达水平，运用MTT法和流式细胞术检测转染后MCF-7细胞增殖水平和凋亡率的变化。将S100A4- shRNA表达载体转染入MCF-7细胞，G418筛选及克隆化培养,并运用划痕实验检测其迁移力的变化。结果经测序鉴定证实成功构建S100A4-shRNA表达载体。转染48h后，所构建的S100A4-shRNA表达载体能够有效抑制MCF-7细胞中S100A4的表达，并且可以显著降低MCF-7细胞增殖水平以及诱导细胞进入晚期凋亡；稳定转染S100A4-shRNA表达载体的MCF-7细胞形态无显著变化，但是迁移力明显下降。结论S100A4-shRNA表达载体能有效地抑制乳腺癌MCF-7细胞中S100A4的表达，从而降低细胞增殖水平和迁移力。

Effects of Short Harpin in RNA Targeting S100A4 Gene on Proliferation and Migration Potential of MCF-7 Cells in vitro

Objective To study the effects of short hairpin in RNA(shRNA) targeting S100A4 on the proliferation and migration potential of breast cancer MCF-7 cells.Methods The S100A4-shRNA expression vector was constructed and confirmed by sequencing. Then the plasmid was transfected into MCF-7 cells via lipofectamine TM 2000, and the changes of S100A4 expression were determined using quantitative RT-PCR and Western blot 48 h after transfection. Flow cytometry and MTT assay were performed to assess the effect of the S...