| 白术茯苓汤调控脾虚大鼠血管活性肠肽总多糖的提取工艺研究 |
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| 引用本文： | 彭腾,贾波,彭成,文跃强,宴永明,张楠,贺小芳.白术茯苓汤调控脾虚大鼠血管活性肠肽总多糖的提取工艺研究[J].成都医学院学报,2008,3(4):253-255. |
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| 作者姓名： | 彭腾 贾波 彭成 文跃强 宴永明 张楠 贺小芳 |
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| 作者单位： | 成都中医药大学,成都,610075 |
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| 摘 要： | 目的研究白术茯苓汤总多糖的提取工艺。方法通过单因素实验及正交实验确定提取总多糖的优化条件，用分光光度法测定总多糖的含量。结果白术茯苓汤总多糖提取的最佳条件是：料液比为1：30、提取时间为2．0h、提取次数为3次、温度90℃．在此条件下测得白术茯苓汤中总多糖含量为48．25mg／g．结论该方法简便、快速、准确，适用于该复方中总多糖的含量测定。
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| 关 键 词： | 白术茯苓汤 总多糖 正交实验 含量测定 |
Study on Extraction of the Polysaccharide in Controlling Rat Spleen Deficiency Vasoactive Intestinal Peptide from Atractylodes Poria Soup |
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| Authors: | PENG Teng JIA Bo PENG Cheng WEN Yue-qiang YAN Yong-ming ZHANG Nan HE Xiao-fang |
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| Institution: | (Chengdu University of TCM, Chengdu 610075,China) |
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| Abstract: | Objective To investigate the extraction process of polysaecharide from Atractylodes Poria soup. Methods The optimal condition was determined by the orthagonal experiment and single-factor test. Polysaecharides were measured by spectrophotometry. Results Experiment reveals that optimal condition for extraction was as fol lows： temperature： 90℃; duration： 2 hours; times： 3 times; ratio： 1：30（w/v）. The volume of polysaccharides in Atractylodes Poria soup is 48.25 mg/g. Conclusion The method is simple, rapid and accurate for determination of polysaceharide from the compound. |
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| Keywords: | Atractylodes Poria soup polysaccharide orthogonal experiment determination |
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