| 急性胰腺炎相关性肺损伤模型的建立 |
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| 引用本文： | 熊玉霞,周业江,孟宪丽,秦大莲.急性胰腺炎相关性肺损伤模型的建立[J].医药论坛杂志,2009(5). |
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| 作者姓名： | 熊玉霞 周业江 孟宪丽 秦大莲 |
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| 作者单位： | 泸州医学院药理教研室;泸州医学院附属医院普外科;成都中医药大学药理教研室; |
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| 基金项目： | 四川省卫生厅资助项目(80168) |
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| 摘 要： | 目的采用5%牛磺胆酸钠逆行胰胆管注射建立急性出血坏死性胰腺炎(AHNP)大鼠模型,动态观察肺组织损伤情况,以评价能否建立有效的急性胰腺炎相关性肺损伤(APALI)动物实验模型。方法SD大鼠随机分成假手术对照组和AHNP模型组,动态观察3,6,12和24h四个时相点的血清淀粉酶、胰腺和肺病理组织学改变、肺组织湿干重比和髓过氧化物酶活性,伊文氏蓝法观察1.5,3和6h三个时相点肺血管通透性。结果血清淀粉酶、肺组织湿干重比和髓过氧化物酶活性在5%牛磺胆酸钠诱导后3h明显增高,并持续到24h,肺组织病理检查发现,牛磺胆酸钠诱导后肺组织就出现了明显的充血、出血、炎症细胞浸润、组织结构破坏等肺组织损伤的病理表现,AHNP大鼠1.5h肺血管通透性就显著增高,且随时间呈显著性增加,各指标与假手术组比较差异有显著性(P<0.05)。结论应用5%牛磺胆酸钠能够成功建立APALI大鼠模型,为进一步研究对APALI的防治作用奠定了基础。
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| 关 键 词： | 急性胰腺炎 肺损伤 大鼠 模型 |
Establishment of Rats Model of Acute Pancreatitis Associated Lung Injury |
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| XIONG Yuxia,ZHOU Yejiang,MENG Xianli,et al.Establishment of Rats Model of Acute Pancreatitis Associated Lung Injury[J].Journal of Medical Forum,2009(5). |
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| Authors: | XIONG Yuxia ZHOU Yejiang MENG Xianli |
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| Institution: | XIONG Yuxia,ZHOU Yejiang,MENG Xianli,et al Department of Pharmacology,Luzhou Medical College,Luzhou 646000,China |
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| Abstract: | Objective To establish a rats model of acute pancreatitis associated lung injury(APALI) by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct.Methods SD rats were randomly divided into sham operation and acute hemorrhagic and necrotic pancreatitis(AHNP) group.The rats were killed at 3,6,12,24 hours after operation.Serum amylase,wet/dry weight ratio and activity of myeloperoxidase of lung were assated.Histopathological alterations of lung was evaluated.Vascular permeability of lung w... |
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| Keywords: | Acute pancreatitis Lung injury Rats Model |
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