Antiproliferative effects of tree-of-heaven (Ailanthus altissima Swingle) |
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Authors: | De Feo Vincenzo Martino Laura De Santoro Angelo Leone Arturo Pizza Cosimo Franceschelli Silvia Pascale Maria |
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Institution: | Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno Via Ponte don Melillo, 84084 Fisciano Salerno, Italy. defeo@unisa.it |
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Abstract: | Tree-of-heaven (Ailanthus altissima Swingle) was evaluated for its cytotoxic and antiproliferative activities by a bioassay-oriented study. Cytotoxicity observed in HeLa cells was time-dependent; the treatment with 10 microg/mL of the root chloroform extract reduced cell viability by 56% at 24 h and 29% at 48 h of exposure, whereas no effect was recorded in the controls. Significant effects were observed also for chromatographic fractions and the pure isolated alkaloid 1-methoxy-canthin-6-one. After 72 h of incubation cell viability was less than 10% for all treatments. A possible apoptotic effect was evaluated by monitoring the presence of hypodiploid elements in HeLa cells as well as in SAOS, U87MG and U-937 tumor cell lines. The cells incubated for different times with the active extract, fraction and pure alkaloid isolated from A. altissima showed a remarkable increase in the apoptosis. |
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Keywords: | Ailanthus altissima cytotoxicity antiproliferative activity apoptosis 1‐methoxy‐canthin‐6‐one |
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