Creatinine determination in the urine of alcaptonuric patients |
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| Authors: | L Sadílek |
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| Affiliation: | 1. State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing 100029, China;2. School of Biological and Environmental Engineering, Zhejiang University of Technology, No. 6 District, Zhaohui, Hangzhou 310032, China;1. UCL Institute of Ophthalmology, University College London, London WC1E 6BT, UK;2. Centre for Mathematics and Physics in Life Sciences and Experimental Biology (CoMPLEX), University College London, London WC1E 6BT, UK;3. Centre for Research in Autism and Education (CRAE), Department of Psychology and Human Development, UCL Institute of Education, University College London, London WC1H 0AL, UK;4. School of Psychology, University of Western Australia, Crawley, Perth, Western Australia 6009, Australia;5. School of Optometry and Vision Science, University of Auckland, Auckland 1010, New Zealand;6. Department of Psychology, Queen Mary University, London E1 4NS, UK;1. Institute of Life Science, Chongqing Medical University, Chongqing, 400016, PR China;2. College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010110, PR China;3. College of Pharmacy, Chongqing Medical University, Chongqing, 400016, PR China;1. College of Information Engineering, North China University of Technology, Beijing 100144, China;2. School of Aviation Science and Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China;3. China Academy of Launch Vehicle Technology, Beijing 100076, China;4. Aviation Key Laboratory of Science and Technology on Aero Electromechanical System Integration, Nanjing 211106, China |
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| Abstract: | If the action of homogentisic acid is neglected, the values of creatinine determined in alcaptonuric urines by Jaffe's reaction are about 50% in excess on the average. Based on these results, misleading clinical conclusions about renal functional state can be drawn.Homogentisic acid interference can be completely avoided by previously isolating creatinine on strongly acid cation-exchanger. A modification of the Teger-Nilsson method for creatinine determination is presented. Optimum conditions for creatinine isolation on the ion-exchange column and the photometric behaviour of the mixture of homogentisic acid and creatinine with alkaline picrate are discussed. |
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