| 药材玉竹RAPD反应体系的优化 |
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| 引用本文： | 林丽美,刘塔斯,龚力民,吴泽宇,胡胜全.药材玉竹RAPD反应体系的优化[J].中南药学,2006,4(5):383-385. |
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| 作者姓名： | 林丽美 刘塔斯 龚力民 吴泽宇 胡胜全 |
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| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700
2. 湖南中医药大学药学院,长沙,410004 |
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| 摘 要： | 目的寻找玉竹的最佳RAPD反应体系，保证实验结果的稳定可靠性。方法采用单因素多水平的梯度实验和正交设计实验相结合，对两种不同实验方法所得结果进行分析。结果确定了适合于玉竹的最佳RAPD反应体系。结论优化的RAPD反应体系是保证实验结果稳定可靠的重要因素之一。
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| 关 键 词： | 玉竹 |
| 文章编号： | 1672-2981（2006）05-0383-03 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006-04-062006-07-14 |
Optimization of random amplified polymorphic DNA reaction system for Yuzhu (Polygonatum Odoratum) |
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| Lin Li-mei,Liu Ta-si,Gong Li-min,Wu Ze-yu,Hu Sheng-quan.Optimization of random amplified polymorphic DNA reaction system for Yuzhu (Polygonatum Odoratum)[J].Central South Pharmacy,2006,4(5):383-385. |
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| Authors: | Lin Li-mei Liu Ta-si Gong Li-min Wu Ze-yu Hu Sheng-quan |
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| Institution: | 1. Institute of Chinese Materia Medical, China Academy of Traditional Chinese Medicine, Beijing 100700; 2. College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410007 |
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| Abstract: | OBJECTIVE To determine the optimal random amplified polymorphic DNA(RAPD) reaction system for medical material Yuzhu(Polygonatum Odoratum) and to ensure stable and reliable experimental results.METHODS The gradient experiments of single factor and multilevel orthogonal design were integrated and the results of the two experiments were analyzed.RESULTS The optimal RAPD reaction system for medical material Yuzhu(Polygonatum Odoratum) was determined.CONCLUSIONS The optimal RAPD reaction system is one of the most important factors for stable and reliable experimental results. |
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| Keywords: | RAPD |
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