Monoclonal antibodies specific for kappa chain, lambda chain, and IgG1 of human gammaglobulin |
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Authors: | S Tonkonogy G Trinchieri B Perussia M Nabholz |
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Affiliation: | 1. Department of Immunology, Swiss Institute for Experimental Cancer Research, Unit of Genetics, Swiss Institute for Experimental Cancer Research, Switzerland;2. Unit of Human Cancer Immunology, Ludwig Institute for Cancer Research, 1066 Epalinges-sur Lausanne, Switzerland |
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Abstract: | Hybrid cell lines secreting antibodies specific for human gammaglobulin (HGG) were prepared by cell fusion and cloning techniques. The monoclonal antibodies were tested for their antibody reacts with a different antigenic determinant of HGG. One reacts with isolated kappa (kappa) light chains, one with isolated lambda (lambda) light chains, and one with the Fc fragment of IgG1 molecules. The reactivity patterns of two additional monoclonal antibodies are more complex. One reacts with a determinant present on the Fc of all IgG subclasses and the other binds to a determinant on the Fab of IgG molecules. The two monoclonal antibodies reactive with light chains also bind to surface components of human B cells. The murine immunoglobulin (Ig) class of each clone product was identified. |
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Keywords: | HGG human gammaglobulin RT room temperature DMEM Dulbecco's modified Eagles medium PBL peripheral blood lymphocytes E erythrocyte PX63 P3-X63-Ag8 murine myeloma cell line E-RFC erythrocyte rosette-forming cell HAT hypoxanthine, aminopterin, and thymidine medium agg HGG heat-aggregated HGG iv intravenously Ig immunoglobulin ip intraperitoneally λ immunoglobulin im intramuscularly κ kappa light chain of Ig DS double stranded PEG polyethylene glycol BSA bovine serum albumin |
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