Surgical Caloric Restriction Ameliorates Mitochondrial Electron Transport Dysfunction in Obese Females |
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Authors: | Jing Li Ritchie J Feuers Varsha G Desai Sherry M Lewis Peter H Duffy Martha A Mayhugh Jr" target="_blank">George CowanJr Cynthia K Buffington |
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Institution: | (1) Guangzhou Institute of Respiratory Disease, Guangzhou, PR China;(2) Divisions of Veterinary Medicine, The National Center for Toxicological Research, Jefferson, AR, USA;(3) Systems Toxicology, The National Center for Toxicological Research, Jefferson, AR, USA;(4) Scientific Coordination, The National Center for Toxicological Research, Jefferson, AR, USA;(5) Genetic and Reproductive Toxicology, The National Center for Toxicological Research, Jefferson, AR, USA;(6) Bionetics, Jefferosn, AR, USA;(7) Department of Surgery, University of Tennessee, Memphis, TN, USA;(8) Florida Hospital Celebration Health, Celebration, FL, USA;(9) Florida Hospital Celebration Health, 400 Celebration Place, Rm A250, Celebration, FL 34747, USA |
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Abstract: | Background The authors examine the mitochondrial electron transport system (ETS) with regard to caloric restriction and body size in
humans.
Methods The study population included 59 morbidly obese (MO) female subjects with mean body mass index (BMI) 49.6 ± 1.7 and 40 age-matched
previously morbidly obese patients with surgically-induced caloric restriction (SCR) and mean BMI 28.9 ± 1.1. ETS function
in the 2 study groups were made by measuring their lymphocyte mitochondrial ETS complexes IIV activities and complex III binding
kinetics. Linear regression analyses were used to analyze the interactions between ETS function and BMI, energy intake, and
metabolic status.
Results The MO, as compared to SCR, subjects had significantly (P < 0.01) higher ETS complexes II-IV activities (complex II = 20.4 ± 1.9 vs 15.3 ± 1.1, complex III = 129.4 ± 10.1 vs 72.3 ± 4.9,
complex IV = 3.1 ± 0.3 vs 1.4 ± 0.1 nmol/mg/min for the MO vs SCR, respectively). ETS complexes activities were positively
and significantly correlated with subjects’ BMI, carbohydrate caloric intake, and fasting plasma insulin levels. Michaelis-Menten
kinetic analysis showed that the Km for ubiquinol-2 in complex III of MO patients was 2-fold greater than SCR values, reflecting
an apparent reduction in substrate binding capacities producing a resistance to electron flow in the MO population. Caloric
consumption, carbohydrate calories, insulin levels, and BMI were also each significantly (P < 0.05) and positively correlated with the Km of Complex III.
Conclusions ETS function and efficiency are compromised by increasing BMI and caloric consumption in morbidly obese women, and caloric
restriction may reduce the potential for excessive oxidative free radical generation via the ETS. |
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Keywords: | Electron transport system caloric restriction morbid obesity lymphocytes mitochondria obesity surgery |
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