采用组织芯片技术检测正常涎腺及其肿瘤组织中Skp2、P27的表达

目的:检测S期激酶相关蛋白2(S-phase kinase-associated protein2,Skp2)和抑癌基因p27在涎腺正常与良恶性肿瘤组织的表达,并探讨其意义。方法:采用组织芯片技术及免疫组化SP法对70例涎腺正常与良恶性肿瘤组织中的Skp2和P27表达进行检测,并做统计学分析。结果:2种良性肿瘤即基底细胞腺瘤与多形性腺瘤之间及2种恶性肿瘤即腺样囊性癌与黏液表皮样癌之间,Skp2与P27表达无显著差异。正常涎腺与良性肿瘤组织的比较,仅Skp2的阳性率有显著性差异;正常涎腺与恶性肿瘤组织的比较及涎腺良性与恶性肿瘤组织的比较,Skp2与P27的阳性率均有显著性差异。结论:Skp2与P27的异常表达可能参与了涎腺肿瘤的发生发展。Skp2蛋白表达与靶蛋白P27蛋白降解增强有关。

Investigation for the expressions of Skp2 and P27 in normal and tumor salivary tissues by tissue chip technique

Objective:To study the expressions of Skp2 and P27 in the normal and tumor tissues of salivary glands,and then to evaluate their significance in the development and progression of salivary tumor.Methods:SP immunohistochemical method was used to evaluate the expression of Skp2 and P27 in the normal and tumor tissues of salivary glands. Results:The rates of Skp2 and P27 expression were neither significantly different between polymorphic adenoma and basal cell adenomas, nor significantly different between adenoid cystic carcinoma and mucoepidemoid carcinoma. The rates of Skp2 positive were significantly different between normal salivary glands and benign salivary tumor, as well as between normal salivary glands and malignant salivary tumor. The rates of Skp2 and P27 positive were significantly different between normal salivary glands and malignant salivary tumor.Conclusion:Skp2 overexpression and decreased expression of P27 might play an important role in the development and progression of salivary tumor, overexpression of Skp2 protein may lead to increase of P27 degradation.