姜黄素阻断NF-κB信号通路抑制人卵巢癌细胞株3AO增殖

目的：探讨姜黄素（curcumin）对体外培养人卵巢癌细胞株3AO细胞增殖抑制及其作用机制.方法：选用人卵巢癌细胞株3AO进行体外培养,以20μg/L、40μg/L、80μg/L姜黄素分别处理3AO细胞24-48h,MTT比色法检测细胞生长活性,流式细胞仪检测细胞周期时相变化,Western blot法检测NF-κB、P-NF-κB、IκB、P-IκB蛋白的变化.结果：姜黄素对人卵巢癌细胞株3AO生长的抑制作用,呈剂量、时间依赖性.流式细胞仪分析证实姜黄素能使3AO细胞周期阻滞于G0/G1期,Western blot结果显示随姜黄素作用浓度的增加,NF-κB、P-NF-κB蛋白表达水平明显下降,IκB、P-IκB蛋白表达没有明显改变.结论：姜黄素对3AO细胞有明显的增殖抑制作用,此作用可以通过下调NF-κB蛋白表达及磷酸化实现,这是姜黄素抑制卵巢癌细胞生长的分子机制之一.

Effects of curcumin on proliferation of human ovarian cancer cell line 3AO in vitro

Objective： To investigate the effects of curcumin on proliferation of human ovarian cancer cell line 3 AO in vitro. Methods：Human ovarian cancer cell line 3AO was cultured in vitro and treated with curcumin of different concentration （20ug/L ,40ug/L, 80ug/L）. The inhibition rates of cells were detected by MTT assay; cell cycle distribution was detected by/low cytometry（FCM） ; expression of NF - kB,p - NF - kB ,IKB,p - IkB in 3AO cell was observed by western bolt. Results ：Curcumin inhibited the proliferation of 3AO cells in a dose -and time -dependent manner. Cell growth was arrested at G0/G1 stage. The expression of NF - kB, p - NF - kB was increased in a dose dependent manner,but the expression of IkB,p- IkB was not affected. Conclusion： Curcumin can inhibits the pro- liferation of 3AO,it is related to the down -regulation of NF- kB and p- NF- kB.