Identification of a novel HLA-DQB1*06 allele, DQB1*0618 |
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Authors: | N Casamitjana J Gil E Campos M Santos N Nogués A Ribera E Palou |
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Institution: | Servei d'Immunohematologia, Centre de Transfusió i Banc de Teixits, Barcelona, Spain. |
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Abstract: | We report here the identification of a novel DQB1*06 allele, DQB1*0618, found in a bone marrow donor. The new allele was detected during routine DNA-based HLA typing by an ambiguous pattern of probe hybridization, obtained by polymerase chain reaction using sequence-specific oligonucleotides (PCR-SSO). Molecular cloning and sequencing confirmed that the new allele is identical to DQB1*0609 at exon 2 except for 3 nucleotide substitutions at positions 353, 356 and 367, also found in other alleles. These nucleotide changes may explain its anomalous reactivity. |
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Keywords: | HLA-DQB1 allele DQB1*0618 nucleotide sequence |
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