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微生物发酵炮制对红花抗氧化活性的影响
引用本文:冯志华,孙启玲,米坤,罗强. 微生物发酵炮制对红花抗氧化活性的影响[J]. 中草药, 2004, 35(6): 630-633
作者姓名:冯志华  孙启玲  米坤  罗强
作者单位:1. 四川大学生命科学学院,四川,成都,610064
2. 乐山师范学院,环境与生命科学系,四川,乐山,614004
基金项目:四川省中医药管理局基金资助项目 ( 2 0 0 2 14 )
摘    要:目的研究地衣芽孢杆菌C2-13发酵炮制对红花抗氧化活性的影响。方法邻二氮菲-Fe^2 氧化法测定羟自由基清除率,比色法测定红细胞的溶血作用、肝脂质过氧化物含量,HPLC法分析发酵产物。结果红花经C2-13的发酵炮制其抗氧化功效显著提高。HPLC分析还观察到红花中一些成分发生了改变。结论红花中一些有效成分可能是经C2-13的生物转化提高了其抗氧化功效。

关 键 词:红花 地衣芽孢杆菌 发酵 炮制 抗氧化
文章编号:0253-2670(2004)06-0630-04
收稿时间:2003-09-19
修稿时间:2003-09-19

Effect on antioxidative activities of dried flower of Carthamus tinctorius processed by microbes fermentation
FENG Zhi-hu,SUN Qi-ling,MI Kun and LUO Qiang. Effect on antioxidative activities of dried flower of Carthamus tinctorius processed by microbes fermentation[J]. Chinese Traditional and Herbal Drugs, 2004, 35(6): 630-633
Authors:FENG Zhi-hu  SUN Qi-ling  MI Kun  LUO Qiang
Affiliation:FENG Zhi-hua~1,SUN Qi-ling~
Abstract:Object Effects of Bacillus licheniformis (Weigmann) Chester C2-13 on antioxidative activities of dried flower of Carthamus tinctorius L. (DFCT) were studied. Methods Clearance of hydroxyl radical was determined by 1, 10-phenanthroline-Fe~(2 ) oxidative assay. The hemolysis of red blood cells, MDA production in hematocytolysis liver homogenate were measured by colorimetric assay. Products of fermentation were analyzed by HPLC. Results C2-13 can increase greatly antioxidative activities of DFCT. Using HPLC assay, it is observed that the fermentation and processing by the strain can improve the effective components of DFCT. Conclusion Some effective components of DFCT may be biotransformed by C2-13, so that the antioxidative activities are promoted.
Keywords:the dried flower of Carthamus tinctorius L. (DFCT)  Bacillus licheniformis (Weigmann) Chester  fermentation  processing  antioxidation  
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