慢性肾病患者治疗前后血浆VEGF和外周血B细胞、T细胞亚群检测的临床意义

目的：探讨了慢性肾病患者治疗前后血浆VEGF和外周血B细胞和T细胞亚群水平的变化及意义。方法：采用酶联免疫法（ELISA）和单克隆抗体法分别测定32例慢性肾病患者治疗前后血浆VEGF和外周血B细胞及T细胞亚群水平的变化，并与35名正常健康人作比较。结果：慢性肾病患者在治疗前血浆VEGF和B细胞数非常显著地高于正常人组（P〈0．01），而CD3、CD4、CD4／CD8比值则显著地低于正常人组（P〈0．01），经治疗后6个月，与正常人比较仍有显著性差异（P〈0．05）。结论：慢性肾病是一种自身调节免疫异常的疾病。

Clinical Significance of Measurement of Changes of Plasma VEGF Levels, Penipheral B-lymphocyte Number and T-cell Subsets After Treatment in Patients with Chronic Nephropathy

Objective To study the significance of changes of plasma VEGF level peripheral B-lymphocyte number and T-cell subsets after treatment in patients with chronic nephropathy.Methods Plasma VEGF(with ELISA)levels,penipheral B-lymphocyte number and T-cell subgsets(with monoclonal antibody technique)were examined in 32 patients with chronic nephropathy both before and after treatment as well as in 35 controls.Results Before treatment,plasma VEGF and B-lymphocyte number were significantly higher in the patients than those in controls(P<0.01).While the CD3,CD4,CD4/CD8 levels were significantly lower(P<0.01).After treatment for 6 months all the levels changes markedy toward normal but remained significantly different from those in controls(P<0.05).Conclusion Chronic nephropathy is a kind of autoimmune diseases with abnormal immuno-regulation.