| 冠心舒通胶囊含药血清对AGEs诱导大鼠血管平滑肌细胞增殖的影响 |
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| 引用本文： | 齐佳昕,赵琳,孔令阁,王明磊,王鹏,任婕,王麟.冠心舒通胶囊含药血清对AGEs诱导大鼠血管平滑肌细胞增殖的影响[J].中药药理与临床,2012(1):155-158. |
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| 作者姓名： | 齐佳昕 赵琳 孔令阁 王明磊 王鹏 任婕 王麟 |
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| 作者单位： | 哈尔滨医科大学附属第五医院心内科;内蒙古民族大学第二附属医院心内科;中国医科大学药理学教研室;哈尔滨医科大学大庆校区生理教研室 |
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| 摘 要： | 目的:探讨冠心舒通胶囊含药血清对晚期糖基化终产物(AGEs)诱导血管平滑肌细胞(VSMCs)增殖的影响及其可能机制。方法:分别以0.5g/kg、1g/kg、2g/kg剂量冠心舒通胶囊内容物对大鼠灌胃给药28d制备冠心舒通胶囊含药血清;以各剂量含药血清单独或联合体外制备的AGE-牛血清白蛋白(AGE-BSA)处理原代培养大鼠VSMCs,MTT法检测各处理组细胞增殖水平、RT-PCR检测细胞血小板源性生长因子-B(PDGF-B)mRNA表达水平、酶联免疫吸附法(ELISA)检测培养上清PDGF-B浓度。结果:40mg/L终浓度AGE-BSA显著增强VSMCs增殖水平(P<0.05),0.5g/kg、1g/kg、2g/kg剂量冠心舒通胶囊含药血清对正常VSMCs生长未见显著影响(P>0.05),但显著抑制AGE-BSA诱导的VSMCs增殖(P<0.05),作用具有明显的剂量依赖性。AGE-BSA诱导VSMCs PDGF-B表达与分泌显著增强(P<0.05),1g/kg、2g/kg剂量冠心舒通胶囊含药血清可有效抑制AGE-BSA这一作用(P<0.05),0.5g/kg剂量血清对AGE-BSA这一作用则未见明显影响(P>0.05)。结论:冠心舒通胶囊可剂量依赖性抑制AGEs诱导的VSMCs增殖,作用机制与抑制AGE诱导的PDGF-B表达与分泌有关。
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| 关 键 词： | 冠心舒通胶囊 晚期糖基化终产物 血管平滑肌细胞 血小板源性生长因子 |
Influence of Guanxin Shutong containing serum on vascular smooth muscle cells proliferation induced by advanced glycation end products |
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| Qi Jiaxin,Zhao Lin,Kong Lingge,Wang Minglei,Wang Peng,Ren Jie,Wang Lin.Influence of Guanxin Shutong containing serum on vascular smooth muscle cells proliferation induced by advanced glycation end products[J].Pharmacology and Clinics of Chinese Materia Medica,2012(1):155-158. |
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| Authors: | Qi Jiaxin Zhao Lin Kong Lingge Wang Minglei Wang Peng Ren Jie Wang Lin |
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| Institution: | 1 Department of Cardiology,the Fifth Affiliated Hospital of Harbin Medical University,Daqing 163319;2 Department of Cardiology,the Second Affiliated Hospital of Inner Mongolia University for the Nationalities,Yakeshi 022150;3 Department of Pharmacology,China Medical University,Shenyang 100010;4 Department of Physiology,Harbin Medical University,Daqing 163319) |
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| Abstract: | |
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| Keywords: | Guanxin Shutong Capsule(冠心舒通胶囊) advanced glycation end products vascular smooth muscle cells platelet-derived growth factor |
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