艾叶油对小鼠的遗传毒理学研究

目的:探讨艾叶油对成年小鼠及胚胎鼠的遗传毒性。方法:采用一次性小鼠灌胃急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、小鼠胚胎肝转移微核试验、小鼠精子畸形试验。结果:艾叶油经小鼠口灌胃给药LD50为4.11ml/kg;艾叶油灌胃剂量2ml/kg时,孕鼠和雄鼠诱发的胚胎肝微核率、骨髓微核率和精子畸形率均较对照组显著升高(P<0.05)。艾叶油灌胃剂量为1ml/kg时,诱发的胚胎肝微核率较对照组显著升高(P<0.05),骨髓微核率与精子畸形率与对照组相比无显著性差异。艾叶油灌胃剂量为0.5ml/kg时,诱发的胚胎肝微核率、骨髓微核率、精子畸形率与对照组相比均无显著性差异。结论:一定剂量的艾叶挥发油对小鼠具有潜在的遗传毒性,并呈剂量-反应关系。

Study on genotoxicity of volatile oil in Artemisia argyi

Objective: To explore genotoxicity volatile oil in Artemisia argyi from Gansu.Methods: Acute oral toxicity test,micronucleus test of adult mice bone marrow cells and fetal mice hepatocytes,and sperm shape abnormality test were carried out.Results: The LD50 in mice acute oral toxicity test was 4.11ml/kg;Compared with control group,the micronucleus rates of bone marrow and fetal hepatic cell and teratospermia number were all significantly increased(P<0.05) with a oral dose of 2ml/kg;Only the micronucleus rate on fetal liver was increased significantly(P<0.05) with the oral dose of 1ml/kg;there was no statistic difference in any of the index with the dose of 0.5ml/kg.Conclusions: A potential dose-related genotoxicity of volatile oil in Artemisia argyi was verified in this research.