羟基红花黄色素A注射剂对家兔血小板聚集、超微结构及血浆GMP-140含量的影响

目的:观察羟基红花黄色素A(HSYA)注射剂对家兔血小板聚集功能及超微结构的影响。方法:采用体内实验法,观察三种剂量的羟基红花黄色素A注射剂(10mg/kg、5mg/kg和2.5mg/kg)对由花生四烯酸(AA,0.35mmol/L)、二磷酸腺苷(ADP,300μmol/L)和血小板活化因子(PAF,3.6nmol/L)诱导的家兔血小板聚集作用的影响,以及血小板超微结构的变化;并单独研究了以PAF为诱导剂的情况下,血浆GMP-140的含量。结果:各剂量HSYA注射剂(10mg/kg、5mg/kg和2.5mg/kg)能抑制AA、PAF诱导的家兔血小板聚集;扫描电镜显示,各剂量HSYA注射剂能减少AA和PAF诱导后的聚集型血小板数量并使树突型血小板突起变少变短;另外,各剂量HSYA注射剂还能降低GMP-140的含量。结论:羟基红花黄色素A注射剂具有显著的抗PAF诱导的血小板聚集作用,抑制血小板的活化,从而为临床抗血小板聚集药物的使用提供更多选择。

The effects of HSYA Injection on platelet aggregation,ultrastructure and the content of GMP-140 in rabbits

Objective: To observe the effects of HSYA injection on platelet aggregation and ultrastructure in rabbits.Methods: In vivo experiments,when AA(0.35mmol/L),ADP(300μmol/L) and platelet activating factor(PAF)(3.6nmol/L) induced respectively,we observed the impacts of HSYA injection(10mg/kg,5mg/kg and 2.5mg/kg) on platelet aggregation,ultrastructural changes of platelets and PAF-induced content of GMP-14.Results: HSYA injection inhibited AA and PAF-induced platelet aggregation(P < 0.05,P < 0.01).Scanning electron microscope showed: HSYA injection could reduce the number of aggregation type plateles which were induced by AA and PAF;and make fewer and shorter processes of dendritic platelets(P < 0.05,P < 0.01).HSYA injection can reduce the content of GMP-140(P < 0.01).Conclusions: HSYA injection can antagonism PAF-induced platelet aggregation,inhibit the activation of platelets and reduce the content of GMP140.Thus for,there will be more choices for clinical using of anti-platelet aggregation drugs.