重楼对小鼠急性肝损伤保护作用的研究

目的:探讨重楼醇提物和水提物对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:小鼠随机分为9组,分别每天给予不同剂量的药物:重楼醇提物高、中、低剂量组(3.9、2.6、1.3 g/kg)、水提组的高、中、低剂量组(7.2、4.8、2.4g/kg)和联苯双酯(0.15 g/kg),正常组和模型组给予生理盐水。7d后,除正常对照组外各组小鼠腹腔注射0.1%CCl4制备急性肝损伤模型,16h后取血检测血清ALT、AST活性;测定肝组织匀浆中MDA含量和SOD、GSH的活性及观察肝脏病理学变化。结果:醇提组高剂量(3.9g/kg)和水提物高、中、低剂量(7.2、4.8、2.4g/kg)组均能降低CCl4所致急性肝损伤小鼠血清ALT、AST活性(P<0.01,P<0.05),降低肝脏MDA的含量、增强SOD和GSH活力(P<0.01,P<0.05),并能明显改善肝组织的病理学损伤。结论:重楼水提物对CCl4所致小鼠急性肝损伤具有较好保护作用。

Study on protective effect of ethanol extract and aqueous extract of Paris ployphylla for acute liver injury in mice

Objective: To study the protective effect of ethanol extract and aqueous extract of Paris ployphylla against CCl4-induced acute liver injury in mice and its possible mechanism.Method: Ninety mice were randomly divided into 9 groups as follows: The drug groups mice were treated with ethanol extract and aqueous extract of Paris ployphylla for 7 day at dose of 3.9,2.6 and 1.3g/kg·d and 7.2,4.8,and 2.4g/kg·d,respectively.At the sametime,the normal control group and the model control group took equivalent amount of 0.9% sodium chloride solution by the same route.And mice in the positive control group were treated with 0.15g/kg ·d biphenyl dimethyl dicarboxylate.After the last administration,all groups except the normal control were injected with 0.1%CCl4 solution to induce acute hepatic injury.Thereafter,the contents of ALT、AST in serum;the activity of MDA,SOD and GSH in the liver were measured and the hepatic histological changes were observed by optical microscope.Results:The contents of ALT,AST in serum was significantly decreased(P<0.01),the content of MDA in the liver was also significantly lowered(P<0.01),and the activity of SOD and GSH in liver was significantly improved after preventive treatment with extract of Paris ployphylla(P<0.01 or P<0.01).Also,aqueous extract of Paris ployphylla(7.2,4.8,2.4g/kg) can ameliorated the hepatic pathological changes.Conclusion: aqueous extract of Paris ployphylla have better protective effects than ethanol extract of Paris ployphylla on acute hepatic injury induced by CCl4 in mice.