桂枝挥发油抗甲型流感病毒作用

目的:观察桂枝挥发油及其主要成分桂皮醛体外对甲型流感病毒A/PR/8/34(H1N1)增殖的影响及对该流感病毒株感染小鼠的治疗作用。方法:MTT法检测受试药物对狗肾传代细胞(MDCK)的半数中毒浓度(TC50)及最大无毒浓度(TC0);血凝法测定甲型流感病毒(H1N1)对MDCK细胞的感染性;MTT法和细胞病变法(CPE)测定两种药物体外抑制流感病毒增殖的有效浓度(IC50)和治疗指数(TI)。计算肺指数及HE染色观察药物对流感病毒(H1N1)感染小鼠的治疗作用。结果:桂枝挥发油和桂皮醛对MDCK细胞的TC50分别为5.38×10-3mg/ml和5.49×10-3mg/ml,TC0均为2.5×10-3mg/ml。桂枝挥发油和桂皮醛能抑制流感病毒在MDCK细胞内的增殖,其IC50分别为5.80×10-5mg/ml与5.31×10-5mg/ml,TI分别为92.82和103.35。桂枝挥发油0.174mg/kg和桂皮醛0.132mg/kg连续灌胃5d,明显降低病毒感染小鼠的肺指数,抑制率分别为26.7%和27.4%,并对病理组织形态有改善作用。结论:桂枝挥发油与桂皮醛体外明显抑制甲型流感病毒(H1N1)在MDCK细胞中的增殖,并对流感病毒株感染小鼠有较好的治疗作用。表明桂枝挥发油及桂皮醛具有抗甲型流感病毒作用,桂皮醛是桂枝挥发油抗病毒效应的主要有效成分之一。

Study on the effect of anti-influenza A virus of the volatile oil of Ramulus Cinnamomi

Objective: To study the effect of anti-influenza A virus(H1N1) of the volatile oil of Ramulus Cinnamomi(VORC) and cinnamaldehyde.Methods: The median toxic concentration(TC 50) and maximum non-toxic concentration(TC0) of VORC and cinnamaldehyde on MDCK cells were detected by MTT assay.The transmissibility of influenza A virus(H1N1) stain adapted mouse lung on MDCK cells were detected by hemagglutinin method.The effect of VORC and cinnamaldehyde in H1N1-infected MDCK cells was evaluated via MTT assay and quantitative measurement of the changes in the Cytopathic effects(CPE).The antiviral effect of VORC and cinnamaldehyde in vivo were evaluated by measuring the lung index and HE staining of lung tissue in mice which infected with virus(H1N1).Results: It was found that the TC 50 of VORC and cinnamaldehyde on MDCK cells were 5.38 × 10-3 mg/mL and 5.49 × 10-3 mg/mL respectively.They showed inhibition effect on virus proliferation after adsorption.Their IC 50 were 5.80 × 10-5 mg/mL and 5.31 × 10-5 mg/mL respectively,and therapeutic index(TI) were 92.82 and 103.35 respectively.VORC and cinnamaldehyde by intragastric administration for 5 days could decrease the lung index and pathology appearance of lung tissue in infected mice.Conclusion: This study demonstrated that VORC and cinnamaldehyde had the effect of anti-influenza A virus(H1N1) both in vitro and in vivo,which cinnamaldehyde maybe the effective compounds of VORC ’s effect of anti-influenza virus.