三叶香茶菜根茎对免疫性肝损伤保护作用及机制研究

目的:三叶香茶菜根茎对BCG+LPS所致肝损伤小鼠的保护作用。方法:采用BCG+LPS诱导的小鼠免疫性肝损伤模型,观察三叶香茶菜根茎对小鼠血清ALT水平的影响,并观察肝组织的病理变化;计算小鼠脾脏和胸腺体重系数,检测血清T-SOD,MDA、TNF-α、IL-6水平,探讨三叶香茶菜保肝作用机制。结果:三叶香茶菜根茎150和100g/kg(以生药量计)对BCG+LPS诱导的免疫性肝损伤小鼠血清中ALT水平具有显著抑制作用,明显减轻肝组织病理损;治疗组小鼠血清T-SOD活力(P<0.05)显著高于肝损伤模组,MDA和IL-6水平均显著低于肝损伤模型组(P<0.05)。结论:三叶香茶菜根茎对免疫性肝损伤具有一定保护作用,机制可能是提高机体的SOD水平,实现清除自由基等抗氧化功能,从而保护生物膜,通过免疫调节作用,下调IL-6等的水平,使免疫炎症反应不会过度,从而减轻机体的免疫炎症损伤,保护肝细胞。

The protective effect and to investigate the mechanism of the roots of the Isodonis ternifoliae on immune liver injury in mice induced by BCG+LPS

Objective: To study on the protective effect and to investigate the mechanism of the roots of the Isodonis ternifoliae on immune liver injury in mice induced by BCG+LPS in order to supply trust worthy basis.Methods: The experimental liver injury model induced by BCG+LPS in mice was applied to observe the effect and the mechanism of the roots of the Herba isodonis ternifoliae on immune liver injury.The activities of ALT in serum and the pathological damages of liver in the mice were observed.The levels of T-SOD,MDA,TNF-α and IL-6 in serum of mice were detected.Results: The roots of the Herba isodonis ternifoliae could markedly inhibit the activities of ALT and the levels of MDA,IL-6(P<0.05),could increased the activities of T-SOD(P<0.05) in blood serum of mice whose livers injured by BCG+LPS,and could markedly release liver tissue injury.Conclusions: The protective effect of the cultivated Herba isodonis ternifoliae on experimental liver injury induced BCG+LPS in mice is significant.The mechanism of the protective effect on injured liver of the Herba isodonis ternifoliae maybe due to its anti-lipid peroxidatuon and adjusting immune action,including decreasing the levels of MDA(P<0.05),increasing the activities of T-SOD(P<0.05),adjusting the levels of,TNF-α,IL-6 in blood serum of mice.