赤芍总苷对大鼠放射性肝纤维化的保护作用及机制

目的:探讨赤芍总苷对大鼠放射性肝纤维化的保护作用及机制。方法:雄性大鼠放射性肝纤维化动物模型,将大鼠随机分为正常对照组、模型组、赤芍总苷组(50 mg/kg、25mg/kg),除正常对照组外,其余各组均制备成放射性肝纤维化模型,造模后各组每天给予相应药物灌胃。于照射后第26周末剖杀大鼠,取血和肝脏标本,ELISA法检测大鼠中TGF-β1、TNF-α、IL-6、HA、PC-Ⅲ、LN含量,碱水法检测大鼠肝脏组织中Hyp含量;光镜下观察肝脏HE染色、MASSON染色病理改变;免疫组化法测肝脏组织TGF-β1、Smad3/4/7蛋白表达情况。结果:与正常对照组比较,模型组大鼠肝脏损伤和胶原纤维增生明显,血清中TGF-β1、TNF-α、IL-6、HA、PC-Ⅲ、LN含量明显增加(P<0.01),肝脏组织中Hyp含量增加(P<0.01),肝脏组织中TGF-β1、Smad3/4/7蛋白表达增加(P<0.01)。与模型组比较,赤芍总苷治疗组(50mg/kg)可显著降低肝纤维化大鼠血清中TGF-β1、TNF-α、IL-6、HA、PC-Ⅲ、LN的含量,同时可降低肝脏组织中Hyp含量及降低肝脏损伤程度及胶原纤维增生程度,还可减少大鼠脏肝组织中TGF-β1和Smad3/4蛋白表达,增加肝脏组织中Smad7蛋白表达。结论:赤芍总苷具有明显的抗肝纤维化作用,其机制可能与其阻断TGF-β1/Smad信号传导通路有关。

Protective effect and mechanism of total paeony glycoside on radiactived hepatic fibrosis in rats

Objective: To study the protection and mechanism of Total paeony glycoside(TPG) on radiactived hepatic fibrosis in rats.Methods: Establish experimental animals’ models of radiactived hepatic fibrosis in male SD rats.The rats were randomly divided into Normal control group,Model group,TPG group(50mg/kg).The effects of TPG were observed on the levels of serum TGF-β1,TNF-α,IL-6,HA,PC-Ⅲ,LN and Hyp in rats liver fibrosis,and on liver histopathological changes as well as collagen hyperplasia formation in liver tissue.Liver samples were collected after experiments and stained by immuninochemistry of TGF-β1 and Smad3,4,7.Results: TPG 50mg/kg can significantly reduce serum TGF-β1,TNF-α,IL-6,HA,PC-Ⅲ,LN and Hyp contents in liver fibrosis of rats,improve the liver pathologic injury,reduce collagen hyperplasia in liver of liver fibrosis rats.TPG 50mg/kg treatment could significantly down-regulated TGF-β1 Smad3/4 expression and up-regulated Smad7 expression.Conclussions: TPG has the protective effect against radiactived hepatic fibrosis in rats.Its mechanism may be related to blocking TGF-β1/Smad signal transduction pathway.