党参、甘草皂苷和黄酮部位对IEC-6细胞迁移的影响

目的:观察党参、甘草的皂苷和黄酮提取部位对小肠上皮细胞(IEC-6)迁移的影响。方法:以回流提取法分别提取党参和甘草的皂苷、黄酮部位,并用D101大孔吸附树脂对各部位进行分离纯化,以分光光度法检测含量。IEC-6细胞种植于6孔板并培养24小时,划痕法造细胞迁移模型,划痕后加入各种受试药,24小时后观察细胞迁移情况。结果:党参和甘草黄酮含量(以芦丁计)分别为41.73%和50.98%;党参皂苷含量(以人参皂苷Re计)为40.57%;甘草皂苷含量(以甘草酸计)为57.01%。50mg/L的党参黄酮部位和甘草黄酮部位能促进细胞迁移(P<0.05);党参和甘草的皂苷部位对细胞迁移无明显影响。结论:党参、甘草黄酮部位有促进IEC-6细胞迁移作用,党参、甘草皂苷部位对IEC-6细胞迁移无明显影响。

Effects of flavonoids and saponins of Radix Codonopsis and Glycyrrhizae on IEC-6 cell migration

Objective:To study effects of flavonoids and saponins of Radix Codonopsis and Glycyrrhiza on IEC-6 cell migration.Methods:Extracts containing flavonoids and saponins of Radix Codonopsis and Glycyrrhiza were obtained by refluxing extraction,respectively,and were separated and purified by D101 macroporous resin to obtain flavonoids fraction and saponins fraction contents of the flavonoids and saponins were determined by spectrophotometry.Cells were implanted in 6-well plates and cultured for 24h.,then scratched in each well to build cell migration model,and cDMEM contain testing agents was added in twenty-four hours later,the migrating cells were counted to screen the effective fraction for promoting cell migration.Results: The content of flavonoids in the Codonopsis flavonoids fraction was 41.73%,and that in the Glycyrrhiza flavonoids fraction was 50.98%;the content of saponins in the Codonopsis saponins fraction was 40.57%,and that in the Glycyrrhiza saponins fraction was 57.01%.Each of the flavonoids fractions(50mg/L) from radix Codonopsis and Glycyrrhizae can promote IEC-6 cell migration(P< 0.05,compared with the control group).The Saponins fractions of Radix Codonopsis and Glycyrrhizae had no significant affect on the cell migration.Conclusion: The flavonoids from radix Codonopsis and Glycyrrhizae can promote IEC-6 cell migration,and the Saponins from radix Codonopsis and Glycyrrhizae have no significant affect on the cell migration.