金属蛋白酶抑制剂BB-94抑制腺样囊性癌侵袭转移的实验

目的观察金属蛋白酶抑制剂BB-94对涎腺腺样囊性癌(adenoidcystic carcinoma,ACC)细胞侵袭、转移能力的抑制作用.方法癌细胞体外侵袭能力用重组基底膜侵袭实验测定,聚丙烯酰胺凝胶电泳(polyacrylamidegel

An experimental study of matrix metalloproteinase inhibitor BB-94 inhibits the invasion and metastasis of the human salivary adenoid cystic carcinoma in vitro and in vivo

OBJECTIVE: To study the inhibitory effect of matrix metalloproteinase inhibitor BB-94 on the invasion and metastasis of salivary adenoid cystic carcinoma in vitro and in vivo. METHODS: Reconstituted basement membrane invasion assay was used to evaluate invasive ability of cancer cells. Type IV collagenase was assessed by PAGE substrate zymography. In addition, the antimetastasis effect of the compound was studied in in vivo experiments using nude mice model of pulmonary metastasis with ACC-M line of human salivary adenoid cystic carcinoma. RESULTS: BB-94, at the concentrations of 10 mumol/L and 100 mumol/L, suppressed the reconstituted basement invasion of ACC-M human salivary adenoid cystic carcinoma by 32.9%, 53.2% and reduced Type IV collagenase activities in the serum-free supernatant of the same cells. The pulmonary metastasis rate of the control and treated groups was 90.0% and 20.0%, respectively (P < 0.01). The lung weight of the control and treated groups was (0.2090 +/- 0.0667) g and (0.1532 +/- 0.0378) g, respectively (P < 0.05). CONCLUSIONS: These results suggest that the matrix metalloproteinase inhibitor BB-94 has a strong inhibitory effect on invasion and metastasis of salivary adenoid cystic carcinoma.