急性髓系白血病患者NK细胞表达及功能变化研究

目的:探讨急性髓系白血病(AML)患者自然杀伤(NK)细胞的抗原表达特征和功能标记物的表达特点.方法:采用多参数流式细胞术检测56例初诊AML患者临床样本以及24例健康对照样本中的NK细胞表面标记物以及其功能指标,包括激活性受体NKG2D、NKP46、DNAM-1和杀伤标记颗粒酶B、穿孔素.结果:56例AML患者参照W...

The Expression and Function of NK Cells in Patients with Acute Myeloid Leukemia

Objective:To explore the expression characteristics of antigens and functional markers of natural killer(NK)cells in patients with acute myeloid leukemia(AML).Methods:Multi-parameter flow cytometry was used to detect NK cell surface markers and their functional indicators in 56 newly diagnosed AML patients and 24 healthy controls,including activating receptors NKG2 D,NKP46,DNAM-1,and killing indicators granzyme B,perforin.Results:Referring to the WHO hematopoiesis and lymph tissue tumor classification criteria,56 cases were roughly divided into three types:AML M₁,M₂,and M₄/M₅.However,there was no differences about NK cells among the three types,so it was no longer subdivided.NK cells were divided into two groups:CD3^-CD56^hiCD16^-(CD56^hiNK)and CD3^-CD56^dimCD16⁺(CD56^dimNK).Compared with CD56^dimNK cell population,except for NKP46,the positive expression levels of NKG2 D and other receptors of CD56^hiNK cells in AML patients decreased(P<0.001).Compared with healthy controls,the proportion of CD56^hiNK cells in AML patients increased,while the number and proportion of NK cells and proportion of CD56^dimNK cells significantly decreased(P<0.05).The proportion of perforin in CD56^hiNK cells significantly increased(P<0.05).The expression of DNAM-1 in CD56^hiNK cells,NKG2 D,DNAM-1,and perforin in CD56^dimNK cells decreased significantly(P<0.05).There was no statistically significant difference in expression of other functional indexes in AML patients compared with corresponding indexes of healthy controls.In addition,the proportion of CD56^hiNK cells was positively correlated with the expression of CD34⁺ in AML(r=0.303).Conclusion:Compared with CD56^dimNK,the ratio of CD56^hiNK and the expression of functional markers in AML patients are lower.Compared with healthy controls,the number and expression ratio of NK cells in AML patients decrease and the expression of functional markers is abnormal,indicating that its function is impaired.