| 免疫荧光技术在皮肤活检组织真菌染色中的应用 |
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| 引用本文： | 陈学超,于永翔,王建文,暴芳芳,周桂芝,陈声利,卢宪梅,刘永霞.免疫荧光技术在皮肤活检组织真菌染色中的应用[J].中国麻风皮肤病杂志,2022,38(2):101-103. |
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| 作者姓名： | 陈学超 于永翔 王建文 暴芳芳 周桂芝 陈声利 卢宪梅 刘永霞 |
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| 作者单位： | 山东第一医科大学附属皮肤病医院(山东省皮肤病医院)，山东省皮肤病性病防治研究所，济南，250022 |
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| 基金项目： | 山东省自然科学基金(编号:ZR2019PH069)。 |
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| 摘 要： | 目的：评价免疫荧光染色法在皮肤活检组织真菌染色中的应用价值。方法：经组织病理和组织培养确诊的43例皮肤真菌病患者对其石蜡包埋切片分别进行免疫荧光染色、PAS染色。结果：43例皮肤真菌病中PAS染色阳性27例（62.8%），免疫荧光染色阳性30例（69.7%），免疫荧光染色法较PAS染色阳性率有所提高，两者差异无统计学意义（P>0.05）。同时两种方法均阴性患者5例，两种方法联合真菌检出阳性率为88.4%。结论：免疫荧光染色是提高皮肤活检组织中真菌检出的重要补充。
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| 关 键 词： | 免疫荧光技术 皮肤真菌病 活检组织 |
Application of immunofluorescent staining method for detection of fungi in dermatopathology |
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| CHEN Xuechao,YU Yongxiang,WANG Jianwen,BAO Fangfang,ZHOU Guizhi,CHEN Shengli,LU Xianmei,LIU Yongxia.Application of immunofluorescent staining method for detection of fungi in dermatopathology[J].China Journal of Leprosy and Skin Diseases,2022,38(2):101-103. |
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| Authors: | CHEN Xuechao YU Yongxiang WANG Jianwen BAO Fangfang ZHOU Guizhi CHEN Shengli LU Xianmei LIU Yongxia |
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| Institution: | Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China |
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| Abstract: | Objective:To evaluate the application of immunofluorescent staining method in detecting the fungi in skin tissues.Methods:A total of 43 formalin-fixed paraffin-embedded tissues which were indentified as fungal infection by pathology and fungal culture were stained with Calcofluor White and Periodic acid-Schiff(PAS)stain.Results:In 43 cases,27 cases(62.8%)were positive of PAS,30 cases(69.7%)were positive of immunofluorescent staining,with no significant difference(P>0.05).There were 5 patients both negitive of immunofluorescent staining and PAS and the positivity rate was 88.4%of two methods combined.Conclusion:Fluorescent staining is an supplement for detection of fungi in skin tissues. |
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| Keywords: | immunofluorescent staining dermatomycosis biospy |
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