拳参-413对心肌肥厚大鼠心肌电学的影响

目的：探讨拳参-413抗心肌肥厚大鼠心律失常可能的作用机制.方法：取清洁级SD雄性大鼠32只,体质量200~260 g,随机分成 ①生理盐水对照组;②心肌肥厚模型组;③ 0.5 mmol/kg的拳参-413组;④ 1.0 mmol/kg的拳参-413组.②③④组大鼠每天背部皮下注射1.0 mg/kg异丙肾上腺素,共10天.对照组每天注射相同体积的生理盐水.10天后给药组每天给予不同浓度的拳参-413,连续7天.观察拳参-413治疗前后大鼠左心室重量指数以及乳头肌跨膜电位的变化.结果：实验结果显示,拳参-413（高、低剂量）治疗后,左心室重量指数明显降低,各组心肌肥厚大鼠右心室乳头肌动作电位时程（APD）复极化各水平均不同程度地长于正常对照大鼠,拳参-413高剂量组APD较模型组比较明显缩短（P〈0.05;P〈0.01）.而去极化最大速率（Vmax）、超射值（OS）和动作电位幅度（APA）以及静息膜电位（RP）水平均无明显变化.结论：心肌肥厚大鼠右心室乳头肌动作电位时程明显延长,拳参-413能够有效的缩短APD减轻心肌肥厚,说明拳参-413具有良好的抗心肌肥厚作用.

Effects of polygonum bistorta.L-413 on myocardial hypertrophy rats

Objective：To investigate the potentially mechanism of Potygonum bistorta. L-413 anti HCM （hypertrophic car- diomyopathy） of rats. Method：32 SD rats weight from 200 ~ 260 g were randomly devided into （1） saline control group, （2）HCM model group, （3）Polygonum bistorta. L-413（0.5 mmol/kg） group and （4）Polygonum bistorta. L-413（ 1 mmol/ kg） group. （2）（3）（4）were subcutaneous injected Iso proterenol for 10 d, while control group was subcutaneous injected sa- line as the same size. Then （2）（3）（4） groups were given different concentrations of Polygonum bistorta. L-413 for the next 7 d. Observe the change of left ventricular weight index and papillary muscle transmembrane potential in rats. Result： The experiment results showed that, after the treatment by Polygonum bistorta. L-413 high and low dosage left ventricular weight index was reduce, The papillary muscle action potential duration （ APD ） of rat with myocardial hypertrophy repo- larization levels were higher than normal control rats, and the ADP of Polygonum bistorta. L-413 （1 mmol/kg） group was reduce obviously, but the maximum rate of depolarization （Vmax） ; the level of overshoot value （OS）, action potential amplitude and resting membrane potential were not change extraordinary. Conclusions：The ventricular papillary muscle action potential duration of rats with myocardial hypertrophy was significantly prolonged; Polygonum bistorta. L-413 can reduce the APD and alleviate Myocardial hypertrophy, this prove that the Polygonum bistorta. L-413 has a good anti-myo- cardial hypertrophy treatment.