缬沙坦对糖尿病肾病大鼠肾小管上皮细胞整合素连接激酶表达的影响

目的：观察缬沙坦对糖尿病肾病（DN）大鼠肾小管上皮细胞整合素连接激酶（ILK）表达的影响，探讨缬沙坦肾脏保护作用的可能机制。方法：30只Wistar雄性大鼠随机分为正常组（n=10）、模型组（n=10）和干预组（缬沙坦30mg·kg^-1·d^-1，n=10）。第8、16周每组各处死5只大鼠，观察大鼠肾脏病理改变以及免疫组化方法检测其肾小管上皮细胞转化生长因子-β1（TGF-β1）、ILK的表达并作半定量分析。结果：（1）DN大鼠肾小管上皮细胞ILK与TGF-β1同向表达增高，随病程进展递增；（2）缬沙坦干预后大鼠肾小管上皮细胞ILK表达较模型组显著降低。结论：（1）DN大鼠肾小管上皮细胞ILK与TGF-β1同向表达增高，提示ILK是肾间质纤维化发展的一个重要因素；（2）缬沙坦可能通过下调ILK来改善肾间质纤维化病变而发挥肾脏保护作用。

Effects of Valsartan on Expression of ILK in Renal Tubular Epithelial Cells of Diabetic Nephropathy Rats

Objective：To observe the effects of valsartan on expression of integrin- linked kinase（ILK）in renal tubular epithelial cells of diabetic nephropathy rats, eucidate the possible renal - protective mechanisms of valsartan. Methods：Thirty male wistar rats were randomly divided into 3 groups： normal control group （ n = 10）, diabetic model group （ n = 10）, and valsartan treatment group （valsartan 30 mg·kg^-1·d^-1, n = 10）. Five rats of each group were killed respectively at 8, 16 weeks. The pathology change and expressions of transforming growth factor （TGF -β1 ） and ILK in renal tubular epithelial cells were detected. Results： （ 1 ）Expressions of TGF-β1 and ILK in renal tubular epithelial cells increased in the diabetic model group in a time - dependent manner. （2） ILK expression in valsartan treatment group was significant lower than in diabetic model rats. Conclusion. （1）ILK expression level in renal tubular epithelial cells of diabetic rats is high, which indicates ILK may be an important factor in the progress of renal interstitial fibrosis in diabetic nephropathy. （2）Valsartan can improve the process of renal tubularinterstitial fibrosis by downregulate the ILK expression.