| 用原位PCR方法检测鼻咽癌组织中的EB病毒 |
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| 引用本文： | 许亮国,杨旭宇,谢鹭. 用原位PCR方法检测鼻咽癌组织中的EB病毒[J]. 中华肿瘤杂志, 2000, 22(1): 17-18 |
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| 作者姓名： | 许亮国 杨旭宇 谢鹭 |
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| 作者单位： | 湖南医科大学卫生部癌变原理重点实验室(许亮国!410078长沙，杨旭宇!410078长沙，谢鹭!410078长沙，姚开泰!410078长沙)，广州中山大学生命科学院(陈曲侯，王珣章) |
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| 基金项目： | 国家自然科学基金!( 3 973 0 2 0 0 ) |
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| 摘 要： | 目的 探讨在鼻咽癌组织石蜡切片标本中原位检测EB病毒 (Epstein Barrvirus,EBV)的更灵敏的方法。方法 用地高辛标记的EBVDNA的BamHI W片段为探针 ,分别用原位杂交、原位PCR的方法检测鼻咽癌组织切片中EBV的分布情况。结果 原位PCR比原位杂交检测EB病毒的方法更灵敏。结论 用原位PCR检测鼻咽癌组织切片中EB病毒不失为一种灵敏的方法。
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| 关 键 词： | 鼻咽肿瘤 爱泼斯坦巴尔病毒 聚合酶链反应 原位杂交 |
Detecting Epstein Barr virus in nasopharyngeal carcinoma tissue using in situ polymerase chain reaction |
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| XU Liangguo,YANG Xuyu,XIE Lu,et al.. Detecting Epstein Barr virus in nasopharyngeal carcinoma tissue using in situ polymerase chain reaction[J]. Chinese Journal of Oncology, 2000, 22(1): 17-18 |
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| Authors: | XU Liangguo YANG Xuyu XIE Lu et al. |
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| Affiliation: | Key Laboratory of Carcinogenesis of Chineses Ministry of Public Health, Hunan Medical University, Changsha, 410078, China. |
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| Abstract: | Objective To develop a sensitive non isotopic method for in situ detection of Epstein Barr virus (EBV) in paraffin embedded section of nasopharyngeal carcinoma (NPC) tissue. Methods The EBV BamHI W fragment labeled with digoxigenin as probe was used to perform in situ hybridization and PCR in situ hybridization (in situ PCR).Results The method of in situ PCR for the detection of EBV in NPC tissue was proved to be much more sensitive than in situ hybridization. Conclusion Hybridization in situ of PCR product is useful to detect EBV in NPC tissues. |
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| Keywords: | Nasopharyngeal neoplasms Epstein Barr virus Polymerase chian reaction In situ hybridization |
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