Anti-hyperglycemic effect of ethanolic leaves extract,hexane and water of Urena lobata on diabetic rats induced by high fructose diets and streptozotocin

OBJECTIVE To investigate anti-hyperglycemic effect of ethanolic leaves extract,hexane and water of Urena lobata on diabetic rat induced by high fructose diets and streptozotocin.METHODS This study uses control group post test only with male spraque dawley rats.Diabetic rats was induced by high fructose diet(HFD)and single dose streptozotocin 25mg·kg-1 bw intra peritoneal.The rat was administrated orally with ethanolic leaves extract,hexane and water of U.lobatain concentrations of 500mg·kg-1 bw for 4weeks.Blood sample were collected from the tail vein at 0,15,30,60 and 120min after oral glucose administration and then blood glucose level were measured using a commercially available glucometer.The integrated area under the postprandial glucose curves(AUCs)was calculated by the trapezoidal method.Insulin serum level examined by rat insulin elisa kits.The data was analyzed using ANOVA test continued with LSD test(P<0.05).RESULTS The oral administration of ethanolic leaves extract,hexane and water of U.lobata were able to decrease glucose AUC about 40%,20% and 60% respectively compared to diabetic group(P<0.05),whereas the insulin level was increased by 2,2and 4-fold(P<0.05),respectively.In diabetic groups,glucose AUC was increased approximately 70% compared to normal group(P<0.05)while the insulin level were decreased by 14-fold(P<0.05).CONCLUSION U.lobataleaves extract could control the increase of blood glucose level after glucose administration by improve of insulin secretion.This effect may be related to active compounds that act as an anti-hyperglycemic and antioxidant in U.lobataleaves extract.