Signaling pathways involved in DNA synthesis and migration in response to lysophosphatidic acid and low-density lipoprotein in coronary artery smooth muscle cells |
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| Authors: | Mayumi Komachi Alatangaole Damirin Enkhzol Malchinkhuu Chihiro Mogi Masayuki Tobo Hideo Ohta Koichi Sato Hideaki Tomura Fumikazu Okajima |
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| Affiliation: | aLaboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi 371-8512, Japan;bDepartment of Biochemistry and Molecular Biology, College of Life Sciences, Inner Mongolia University, Huhhot, Inner Mongolia 010021, China;cResearch Laboratory, Kirin Brewery Co., LTD., 3 Miyahara, Takasaki 370-1295, Japan |
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| Abstract: | Low-density lipoprotein (LDL) and lysophosphatidic acid (LPA), one of the lipid components of lipoprotein, induced the DNA synthesis of coronary artery smooth muscle cells (CASMCs). The LDL- and LPA-induced DNA synthesis was markedly inhibited by the LPA receptor antagonist Ki16425, pertussis toxin, small interfering RNAs targeted for LPA1 receptors, and a potent calcineurin inhibitor cyclosporine A. It has been reported that LDL and LPA induced a migration response in a manner sensitive to Ki16425, pertussis toxin, and a LPA1 receptor-specific small interfering RNA. However, cyclosporine A was ineffective in inhibiting the migration response. Instead, an epidermal growth factor (EGF) receptor tyrosine kinase inhibitor markedly suppressed the migration response to LDL and LPA without having any significant effect on DNA synthesis. Thus, the LDL-induced stimulation of DNA synthesis and migration in CASMCs is mediated by its component LPA through LPA1 receptors and Gi/o-proteins. Ca2+/calcineurin pathways and transactivation of EGF receptors mediate LPA1-receptor-induced DNA synthesis and migration, respectively. |
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| Keywords: | Lysophosphatidic acid Low-density lipoprotein Ki16425 Vascular smooth muscle cell |
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