血清微小RNA作为早期乳癌诊断标志物的价值 |
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引用本文: | 程晓鸥,张金玉,葛银林,郑征,李振凤,侯琳.血清微小RNA作为早期乳癌诊断标志物的价值[J].康复与疗养杂志,2014(5):407-410. |
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作者姓名: | 程晓鸥 张金玉 葛银林 郑征 李振凤 侯琳 |
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作者单位: | 青岛大学医学院生物化学与分子生物学教研室,山东青岛266021 |
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基金项目: | 青岛市科技局资助项目(08-2-1-4-nsh); 山东省自然科学基金资助项目(ZR2012HM089) |
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摘 要: | 目的探讨血清5种微小RNA(miRNA)在乳癌早期诊断中作为生物标记物的价值。方法根据生物信息学数据库miRWalk的预测,选定5种与乳癌相关的miRNA(包括miR-31、miR-107、miR-155、miR-200c及miR-205)。采用实时荧光定量PCR检测5种miRNA在42例健康女性、66例乳癌病人中的相对表达量,根据受试者工作特征曲线(ROC曲线)下面积分析诊断乳癌的特异度和灵敏度。结果血清中miR-31、miR-107和miR-200c联合检测诊断乳癌的灵敏度为78.6%,特异度为88.7%。结论血清中miR-31、miR-107和miR-200c的组合有可能成为乳癌早期诊断的新指标。
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关 键 词: | 微RNAs 乳房肿瘤 生物学标记 早期诊断 |
THE VALUE OF SERUM MICRORNAS AS BIOMARKERS FOR EARLY DIAGNOSIS OF BREAST CANCER |
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Authors: | CHENG Xiaoou ZHANG Jinyu GE Yinlin ZHENG Zheng LI Zhenfeng HOU Lin |
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Institution: | (Department of Biochemistry and Molecular Biology, Qingdao University Medical College, Qingdao 266021, China) |
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Abstract: | Objective To assess the value of five serum microRNAs(miRNA)as biomarkers for early diagnosis of breast cancer. Methods According to bioinformatics database miRWalk prediction,five breast-cancer-related miRNAs(miR-31,miR-107,miR-155,miR-200 cand miR-205)were selected.The expressions of the five miRNAs in serum samples of 42 normal controls and 66 breast cancer patients were detected by real-time quantitative PCR.Specificity and sensitivity were analyzed by the areas under the receiver operating characteristic(ROC)curves of these miRNAs. Results The sensitivity and specificity of combined miR-31,miR-107 and miR-200 cfor breast cancer diagnosis were 78.6%and 88.7%,respectively. Conclusion The combination of serum miR-31,miR-107 and miR-200 cmay become a new marker for early diagnosis of breast cancer. |
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Keywords: | microRNAs breast neoplasms biological markers early diagnosis |
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