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Functional detection of the original generation of hippocampal cells planted on to the micro-fluidic chip with artificial neuronal network using the patch clamp recording technique: a preliminary study |
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| Authors: | Xianmin Kong Shanshan Tian |
| Institution: | 1. School of Life Science and Bioengineering, Beijing University of Technology, Beijing, People’s Republic of China;2. Laser Institute Beijing University of Technology, Beijing, People’s Republic of China |
| Abstract: | Purpose: The design of pattern that limits the hippocampal cells growth is an important explore for realizing a simplified artificial neuronal network in vitro. Materials and methods: In this study, we examined the pattern in micro-fluidic chip to stipulate the hippocampal cells adhesion, growth and the formation of a functional neuronal network in vitro. Patch clamp recording technique was used to detect the growth situation and biological function of the haippocampal cells on the micro-fluidic chip which could simulate environment in vivo. Results: We showed that the number of neurons cultured was about 5000–6000 cells on the micro-fluidic chip, which was conductive to the hippocampal cells growth. The result of patch clamp recording technique showed the signals of sodium and potassium channels, meanwhile, it also revealed the signals of synaptic connection. Conclusions: These findings involve placing cells in specific locations to create organized structures, and explore the spread function of synaptic on the micro-fluidic chip. |
| Keywords: | Hippocampal cells artificial neuronal network micro-fluidic chip patch clamp recording technique |