| 沉默信息调节因子1对缺氧心肌细胞凋亡和自噬的影响 |
| |
| 引用本文： | 沈丹凤,刘克坚,王燕,蒋华. 沉默信息调节因子1对缺氧心肌细胞凋亡和自噬的影响[J]. 微循环学杂志, 2019, 0(3): 11-15 |
| |
| 作者姓名： | 沈丹凤 刘克坚 王燕 蒋华 |
| |
| 作者单位： | 四川省第四人民医院心内科;石河子大学医学院第一附属医院心内科;新疆维吾尔自治区人民医院内分泌科;新疆医科大学第一附属医院心内科 |
| |
| 摘 要： | 目的:分析沉默信息调节因子1(Sirt1)对缺氧条件下心肌细胞凋亡和自噬的影响。方法:将心肌H9c2细胞分为Control组、Hypoxia组(缺氧处理24h)、NC+Hypoxia组(转染阴性对照并缺氧处理24h)和Sirt1+Hypoxia组(转染Sirt1过表达载体并缺氧处理24h)。以qRT-PCR和Western blot测定心肌细胞中Sirt1 mRNA和蛋白表达水平,MTT方法评估细胞存活率,流式细胞术测定细胞总凋亡水平,Western blot检测凋亡蛋白Bax、Cleaved Caspase-3水平和自噬蛋白LC3-Ⅱ/Ⅰ、Beclin-1水平。结果:与Control组比较,Hypoxia组细胞中Sirt1 mRNA和蛋白表达水平降低,细胞存活率降低,细胞凋亡率和Bax、Cleaved Caspase-3蛋白水平升高,LC3-Ⅱ/Ⅰ、Beclin-1蛋白水平降低(P<0.05)。与NC+Hypoxia组比较,Sirt1+Hypoxia组细胞中Sirt1 mRNA和蛋白表达水平升高,细胞存活率升高,细胞凋亡率和Bax、Cleaved Caspase-3蛋白水平降低,LC3-Ⅱ/Ⅰ、Beclin-1蛋白水平升高(P<0.05)。结论:Sirt1可抑制缺氧心肌细胞凋亡并诱导细胞自噬。
|
| 关 键 词： | 心肌细胞 沉默信息调节因子1 自噬 凋亡 缺氧 |
Effects of Silencing Sirt1 on Apoptosis and Autophagy of Hypoxic Cardiomyocytes |
| |
| SHEN Dan-feng,LIU Ke-jian,WANG Yan,JIANG Hua. Effects of Silencing Sirt1 on Apoptosis and Autophagy of Hypoxic Cardiomyocytes[J]. Chinese Journal of Microcirculation, 2019, 0(3): 11-15 |
| |
| Authors: | SHEN Dan-feng LIU Ke-jian WANG Yan JIANG Hua |
| |
| Affiliation: | (Deparment of Cardiology,Sichuan Provincial Fourth People’s Hospital,Chengdu 610016,China;Department of Cardiology,The First Affiliated Hospital of the Medical College,Shihezi University,Shihezi 832008,China;Department of Endocrindogy,People's Hospital of Xinjiang Uygur Autonomous Region,Wulumuqi 830001,China;Department of Cardiology,The First Affiliated Hospital of Xinjiang Medical University,Wulumuqi 830054,China) |
| |
| Abstract: | SHEN Dan-feng;LIU Ke-jian;WANG Yan;JIANG Hua(Deparment of Cardiology,Sichuan Provincial Fourth People’s Hospital,Chengdu 610016,China;Department of Cardiology,The First Affiliated Hospital of the Medical College,Shihezi University,Shihezi 832008,China;Department of Endocrindogy,People's Hospital of Xinjiang Uygur Autonomous Region,Wulumuqi 830001,China;Department of Cardiology,The First Affiliated Hospital of Xinjiang Medical University,Wulumuqi 830054,China) |
| |
| Keywords: | Cardiomyocytes Sirt1 Autophagy Apoptosis Hypoxia |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
