加味逍遥散对HPRL大鼠多巴胺受体cAMP/PKA信号转导通路的研究

目的： 探讨以肝脾为核心，运用逍遥散加减方治疗实验性高催乳激素血症（HPRL）模型大鼠下丘脑多巴胺受体环磷酸腺苷（cAMP）/蛋白激酶A（PKA）信号转导通路机制的影响。 方法： 腹腔注射甲氧氯普胺，制作HPRL大鼠模型。随机分成6组：空白对照组、阴性对照组、阳性对照组（溴隐亭组）、逍遥散加减方高、中、低剂量组。给药30 d后，采用免疫组化法测定大鼠下丘脑PKA阳性表达的数量；采用酶联免疫的法测定大鼠血清cAMP的含量、采用免疫组化法及RT-PCR检测大鼠下丘脑多巴胺D1，D2受体。 结果： 与阴性对照组相比较，逍遥散加减各剂量组下丘脑PKA活性明显降低（P < 0.01）；下丘脑cAMP活性显著降低 （P < 0.01）、下丘脑多巴胺D1受体数量有增高趋势、D2受体数量显著增加（P < 0.01）。 结论： 逍遥散加减方对能通过多巴胺受体cAMP-PKA信号转导通路调节泌乳素水平。

Effect of Xiaoyaosan on Dopamine Receptor cAMP/PKA Signal Transduction Pathway in HPRL Rats

Objective: To investigate effect of of Xiaoyaosan on dopamine receptor cyclic adenosine monophosphate(cAMP)/proteinkinase A(PKA) signal transduction pathway in hyperprolactinemia(HPRL) rats. Method: The metoclopramide was injected to establish the animal model,48 SD rats were randomized into 6 groups:control group, model group, bromocriptine group, high dose modified Xiaoyaosan group, middle dose modified Xiaoyaosan group, low dose modified Xiaoyaosan group. The intervention period lasted 30 days. The immune histochemistry method to observe expression of PKA,D1R,D2R,ELISA method to observe expression of cAMP, fluorescent quantitative PCR method was used to observe expression of D1R and D2R. Result: Compared with the control group, modified Xiaoyaosan groups reduced PKA,cAMP activity(P < 0.01);dopamine D2 receptor was significantly increased(P < 0.01). Conclusion: The modified Xiaoyaosan can regulate the PRL level through dopamine receptor cAMP/PKA signal transduction pathway.