| Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum |
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| 引用本文： | 王旗,李英杰. Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J]. 中国人民解放军军医大学学报, 1988, 0(2) |
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| 作者姓名： | 王旗 李英杰 |
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| 作者单位： | Department of Malaria Immunology First Medical College of PLA，Guangzhou，Department of Malaria Immunology，First Medical College of PLA，Guangzhou |
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| 摘 要： | Supernatants collected from the short term in vitro serum-free cultures ofPlasmodium falciparum (Pf) schizont-infected erythrocytes were detected and analysed bycounterimmuno-electrophoresis (CIE) and enzyme-linked immunoabsorbent assay (ELISA).Soluble antigens were purified from the supernatants by immunoabsorbent chroma-tography. It was found that soluble antigens similar to the sonicated Pf antigens inimmunospecificity were present in the supernatants and no erythrocyte membrane antigenswere detectable in the affinity-purified supernatant antigens (Ps-Ag).
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Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum |
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| Wang Qi Li Yingjie. Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J]. Journal of Medical Colleges of PLA(China), 1988, 0(2) |
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| Authors: | Wang Qi Li Yingjie |
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| Affiliation: | Wang Qi Li Yingjie Department of Malaria Immunology,First Medical College of PLA,Guangzhou |
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| Abstract: | Supernatants collected from the short term in vitro serum-free cultures of Plasmodium falciparum (Pf) schizont-infected erythrocytes were detected and analysed by counterimmuno-electrophoresis (CIE) and enzyme-linked immunoabsorbent assay (ELISA). Soluble antigens were purified from the supernatants by immunoabsorbent chroma- tography. It was found that soluble antigens similar to the sonicated Pf antigens in immunospecificity were present in the supernatants and no erythrocyte membrane antigens were detectable in the affinity-purified supernatant antigens (Ps-Ag). |
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| Keywords: | Plasmodium falciparum culture supernatant soiuble antigens antigen purification antigen analysis |
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