告达庭激活Caspase-3诱导人胃癌细胞SGC-7901凋亡

[目的]评价告达庭体外诱导人胃癌细胞SGC-7901凋亡的作用。[方法]MTT法检测细胞活性；吖啶橙染色观察核形态；流式细胞术检测细胞凋亡率：Western-blot及比色法检测Cas-pase-3表达及活性。[结果]告达庭抑制SGC-7901细胞的活性，具有浓度、时间依赖性；告达庭70μM能使SGC-7901细胞出现核染色体浓聚等典型的凋亡形态学改变，告达庭35、70μLM使细胞凋亡率升高，并使Caspase-3蛋白表达量和蛋白活性显著升高。[结论]告达庭可能通过激活Caspase-3诱导SGC-7901细胞凋亡。

Caudatin Induced Apoptosis of Gastric Cancer Cell Line SGC-7901 by Activation of Caspase-3

[ Purpose ] To investigate the apoptosis inducing effects and molecular mechanisms of Cau- datin in gastric cancer cell SGC-7901. [Methods] SGC-7901 cells were cultured in vitro and treated with various concentrations of Caudatin. Cell proliferation was examined by MTr assay and apoptosis were detected by Flow cytometry. Morphological changes were detected by acridine orange staining. The protein expression of caspase-3 was detected by Western blot analysis and the activity of cas- pase-3 was detected by Colorimetric assay. [Results] Caudatin inhibited the growth of SGC-7901 cells in a time and concentration-dependent manner. Caudatin could also induce cell nuclear mor- phological changes,and increase the expression and activity of caspase-3.[Conclusion] Caudatin may inhibit cell proliferation and induce the apoptosis of SGC-7901 by activating caspase-3.