Selection of physiological spermatozoa during intracytoplasmic sperm injection |
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| Authors: | B. Torki‐Boldaji M. Tavalaee M. Bahadorani M. H. Nasr‐Esfahani |
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| Affiliation: | 1. Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;2. Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran;3. Isfahan Fertility and Infertility Center, Isfahan, Iran |
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| Abstract: | Sperm genomic integrity has a significant effect on intracytoplasmic sperm injection (ICSI) outcomes, especially post‐implantation. Spermatozoa selected based on motility and morphology do not guarantee the genomic integrity of spermatozoa. Nearly fifty percentage of spermatozoa in infertile men with normal morphology present different degrees of DNA fragmentation. However, capacitated or hyperactivated spermatozoa show lower degrees of DNA fragmentation. Therefore, selection of hyperactivated spermatozoa may improve ICSI outcome. Routinely, for ICSI, fast‐moving spermatozoa with A or B motility pattern are mainly selected for injection. The result of this study shows that in processed semen samples, hyperactivated spermatozoa are mainly observed in B motility pattern while, in viscous medium like polyvinylpyrrolidone (PVP), hyperactivated spermatozoa are mainly present in spermatozoa with C pattern of motility (nonprogressive). Therefore, we propose spermatozoa with C motility pattern which contains the main population of physiological or hyperactivated spermatozoa should be selected for ICSI. |
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| Keywords: | Capacitation hyperactivation motility intracytoplasmic sperm injection polyvinylpyrrolidone spermatozoa+ |
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