Protective effects of Urtica dioica L. on experimental testicular ischaemia reperfusion injury in rats |
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| Authors: | C. Aktas M. Erboga Z. Fidanol Erboga Y. Bozdemir Donmez B. Topcu A. Gurel |
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| Affiliation: | 1. Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;2. Technology Research and Development Application and Research Center, University of Trakya, Edirne, Turkey;3. Department of Biostatistics, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey;4. Department of Biochemistry, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey |
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| Abstract: | In this study, it was aimed to examine the effects of Urtica dioica L. (UD) that has antioxidant feature in the experimental testicular I/R model in rats in terms of anti‐apoptotic and antioxidative effects. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R + UD (2 mg kg?1) group. Seminiferous tubule calibre measurement, Johnson score, haematoxylin–eosin staining, proliferative cell nucleus antigen (PCNA) immunohistochemical staining and TUNEL as histopathological have been conducted. The structural deterioration in the testicular on I/R group has reduced after the treatment of UD. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end labelling (TUNEL), and there was a rise in the expression of proliferating cell nuclear antigen (PCNA) in testis tissues of UD‐treated rats in the I/R group. The I/R + UD group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that protective effects of UD on the I/R testicles are via reduction of histological damage, apoptosis, oxidative stress and lipid peroxidation. |
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| Keywords: | ischaemia and reperfusion testis‐apoptosis‐oxidative stress Urtica dioica L. |
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