血管内皮生长因子及环氧合酶-2在子宫内膜腺癌中表达及意义

目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）和环氧合酶-2（cyclooxygenase-2,COX-2）在子宫内膜腺癌中表达及临床意义。方法萎缩性正常子宫内膜患者12例（对照组）,子宫内膜增生症患者14例（子宫内膜增生组）,子宫内膜腺癌患者30例（子宫内膜腺癌组）,3组均手术切除子宫内膜组织标本,采用RT-PCR法检测子宫内膜组织COX-2及VEGF mRNA相对表达量。结果子宫内膜腺癌组、子宫内膜增生组、对照组中COX-2mRNA相对表达量分别为（133.58±56.96）%、（56.68±41.75）%、（8.33±5.21）%,VEGF mRNA相对表达量分别为（143.28±59.86）%、（57.33±46.78）%、（15.31±10.86）%,各组间COX-2mRNA及VEGF mRNA相对表达量比较差异有统计学意义（P〈0.01）;子宫内膜腺癌组COX-2表达与VEGF表达呈正相关（r=0.289,P=0.014）。结论COX-2及VEGF在子宫内膜腺癌中均呈高表达,其作为肿瘤恶化程度标记物可为临床诊疗工作提供参考依据。

Expression and significance of vascular endothelial growth factor and cyclooxygenase-2 in endometrial adenocarcinoma

Objective To investigate the expression and significance of vascular endothelial growth factor（VEGF）and cyclooxygenase-2（COX-2）in endometrial adenocarcinoma.Methods Fifty-six specimens of endometrium from 12 cases of atrophic endometrium（control group）,14 of endometrial hyperplasia（hyperplasia group）and 30 of endothelial adenocarcinoma（adenocarcinoma group）were determined the relative expression levels of COX-2and VEGF by RT-PCR technique.Results The relative expressions of COX-2 mRNA were（133.58±56.96）%,（56.68±41.75）% and（8.33±5.21）%,and of VEGF mRNA were（143.28±59.86）%,（57.33±46.78）% and（15.31±10.86）% in adenocarcinoma group,hyperplasia group and control group respectively,showing significant differences among groups（P〈0.01）.COX-2was positively correlated with VEGF in adenocarcinoma group（r=0.289,P=0.014）.Conclusion Both COX-2and VEGF are highly expressed in endothelial adenocarcinoma,and can provide a basis for clinical diagnosis and therapy as markers for tumor progression degree.