晚期糖基化终产物受体在糖尿病足患者中的表达特征

目的:研究晚期糖基化终产物受体(receptor for advanced glycation end products,RAGE)在糖尿病足患者中的蛋白表达水平.方法:按照病例入选标准收集25例糖尿病足患者,21例单纯糖尿病患者,以及20例非糖尿病手术患者,用 ELISA 检测血清 RAGE 表达,取各组患者足部皮肤组织标本,用Western blot检测组织中 RAGE蛋白的表达.结果:RAGE在正常血清、单纯糖尿病患者血清、糖尿病足患者血清及足部皮肤组织中均有表达;糖尿病足患者血清中 RAGE 表达( 313.34 ± 24.36) ng /L 明显高于正常对照组( 59.14 ± 23.71) ng /L及单纯糖尿病组( 86.47 ± 29.35) ng /L.糖尿病足患者足部皮肤组织中RAGE蛋白表达亦明显高于单纯糖尿病患者组及正常对照组,两组间差异有统计学意义( P ＜ 0.05).结论:RAGE 在糖尿病足患者中表达明显升高;RAGE可能与糖尿病足的发生、发展有密切关系;RAGE 可能成为一个新的糖尿病足治疗靶点,对于糖尿病足的临床治疗有积极意义.

Characteristics of Receptor for Advanced Glycation End Products Expression in Patients with Diabetic Foot

Objective: To investigate the expression of the receptor for advanced glycation end products(RAGE) in protein levels of diabetic foot(DF)patients.Method: 25 patients of diabetic foot were collected in accordance with inclusive criteria,21 cases of patients with diabetes alone,and 20 cases surgical patients with out diabetes.The expression levels of RAGE in serum were detected by the method of ELISA.The foot skin tissue samples of patients were collected,and the RAGE protein expression levels of foot skin tissues were detected by Western blot.Result: In the serum of all patients,the RAGE were positive;and it could be found in both normal skin and diabetic foot ulcer tissues of non-diabetic and diabetic patients.The expressions of RAGE in the serum of DF patients were significant higher than other two groups.The protein expressive levels of RAGE in foot ulcer tissues of DF patient were significant higher than in the skin of other two groups.Conclusion: The RAGE expression in DF patients was significantly higher,it may be closely related to the occurrence and development of DF ulcer.RAGE may be a new therapeutic target for DF patient,and it has a positive meaning for the clinical treatment of DF.