慢性单纯性苔藓患者单个核细胞凋亡与细胞因子IGF-1的关系

目的:探讨慢性单纯性苔藓患者单个核细胞凋亡的变化,并检测周围血中细胞因子、白细胞介素-9、白细胞介素-6、肿瘤坏死因子-а和胰岛素样生长因子1的变化,探讨其对MNCs凋亡的影响。方法:采用流式细胞术检测和38例慢性单纯性苔藓患者与10例健康对照者MNCs的凋亡情况。酶联免疫吸附试验(ELISA)检测细胞因子水平。结果:慢性单纯性苔藓患者MNCs凋亡率明显高于健康对照组,患者周围血中所测因子IL-8、TNF-а、IL-6水平均高于正常对照组。细胞因子、IGF-1升高的水平与MNCs的凋亡比例成正相关。结论:慢性单纯性苔藓患者MNCs凋亡增加。存在免疫功能紊乱,炎性细胞因子、IGF-1产生过多可能是导致MNCs凋亡增加的重要机制,通过适度调控细胞因子、IGF-1的分泌和MNCs凋亡有可能会改善慢性单纯性苔藓预后。

Relationship between the Apoptosis of Mononuclear Cell and the Expression of Cytokines and Insulin-like Growth Factor-1 in Patients with Lichen Simplex Chronicus

Objective: To observe the changes of mononuclear cell(MNCs) apoptosis and the level of inflammatorycy tokines and IGF-1 in patients with lichen simplex chronicus,and to study the relationship between them.Method: The apoptosis of MNCs in 38 patients with lichen simplex chronicus and 10 healthy controls were determined with flow cytometry.The inflammatory cytokines and the IGF-1 were tested with ELISA method.Result: The percentage of MNCs apoptosis in the patients with lichen simplex chronicus was significantly higher than that of healthy controls.The levels of IL-8,TNF-а,IL-6,IGF-1 in patients with lichen simplex chronicus were significantly higher than those of healthy controls,and the increased levels o f inflammatory cytokines and IGF-1 in lichen simplex chronicus patients were positively correlated to the percentage of MNCs apoptosis.Conclusion: The data demonstrates that there is a promote apoptotic process of MNCs inactive lichen simplex chronicus.The high expression of inflammatory cytokines and IGF-1 may play an important role in promoting MNCs apoptosis which reduces the functional life span of MNCs.For this reason,modulating the expression of cytokines,IGF-1 and moderately controlling the apoptosis of MNCs may be a new therapeutic target for lichen simplex chronicus treatment.